Blebbing, free Ca2+ and mitochondrial membrane potential preceding cell death in hepatocytes
- 1 January 1987
- journal article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 325 (6099), 78-81
- https://doi.org/10.1038/325078a0
Abstract
Cell surface 'blebbing' is an early consequence of hypoxic and toxic injury to cells. A rise in cytosolic free Ca2+ has been suggested as the stimulus for bleb formation and the final common pathway to irreversible cell injury. Here, using digitized low-light video microscopy, we examine blebbing, cytosolic free Ca2+, mitochondrial membrane potential and loss of cell viability in individual cultured hepatocytes. Unexpectedly, we found that after 'chemical hypoxia' with cyanide and iodoacetate, cytosolic free Ca2+ does not change during bleb formation or before loss of cellular viability. Cell death was precipitated by a sudden breakdown of the plasma membrane permeability barrier, possibly caused by rupture of a cell surface bleb.Keywords
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