A Note on the Staining of the Skeleton of Cleared Specimens with Alizarin Red S
- 1 January 1926
- journal article
- research article
- Published by Informa UK Limited in Stain Technology
- Vol. 1 (4), 123-124
- https://doi.org/10.3109/10520292609115636
Abstract
A selective, progressive method for staining the skeleton in cleared specimens, developed with rat material. Fix in 95% alcohol for at least 48 to 96 hrs. Even longer fixation is desirable. Then place in a 1% solution of KOH until the bones are clearly visible through the surrounding tissues. Transfer directly to a dilute solution of alizarin in KOH, one part alizarin to 10,000 parts of 1% KOH. Allow the stain to act until the desired intensity is attained. Fresh stain may be added if necessary. Complete the clearing process, (1) in Mall's solution, water 79 parts, glycerine 20 parts and KOH 1 part; (2) in increased concentrations of glycerine. Store in pure glycerine. The success of the method depends on obtaining the proper degree of clearing before staining. If the specimen is insufficiently cleared, a general staining of all tissues usually occurs.Keywords
This publication has 3 references indexed in Scilit:
- The differential staining of bone. I. The staining of preserved specimensThe Anatomical Record, 1921
- On ossification centers in human embryos less than one hundred days oldJournal of Anatomy, 1906
- Grundriss der Entwicklungsgeschichte des Menschen und der Säugethiere : für Studirende und ÄrztePublished by Biodiversity Heritage Library ,1897