Nitrous oxide production in soil isolates of nitrate‐ammonifying bacteria
- 31 October 2011
- journal article
- Published by Wiley in Environmental Microbiology Reports
- Vol. 4 (1), 66-71
- https://doi.org/10.1111/j.1758-2229.2011.00302.x
Abstract
Here we provide the first demonstration of the potential for N2O production by soil-isolated nitrate-ammonifying bacteria under different C and N availabilities, building on characterizations informed from model strains. The potential for soil-isolated Bacillus sp. and Citrobacter sp. to reduce NO3-, and produce NH4+, NO2- and N2O was examined in batch and continuous (chemostat) cultures under different C-to-NO3- ratios, NO3--limiting (5 mM) and NO3--sufficient (22 mM) conditions. C-to-NO3- ratio had a major influence on the products of nitrate ammonification, with NO2-, rather than NH4+, being the major product at low C-to-NO3- ratios in batch cultures. N2O production was maximum and accompanied by high NO2- production under C-limitation/NO3-sufficiency conditions in chemostat cultures. In media with lower C-to-NO3-N ratios (5- and 10-to-1) up to 2.7% or 5.0% of NO3- was reduced to N2O by Bacillus sp. and Citrobacter sp., respectively, but these reduction efficiencies were only 0.1% or 0.7% at higher C-to-NO3- ratios (25- and 50-to-1). As the highest N2O production did not occur under the same C-to-NO3- conditions as highest NH4+ production we suggest that a re-evaluation may be necessary of the environmental conditions under which nitrate ammonification contributes to N2O emission from soilKeywords
This publication has 31 references indexed in Scilit:
- Gross Nitrogen Transformations and Related Nitrous Oxide Emissions in an Intensively Used Calcareous SoilSoil Science Society of America Journal, 2009
- Nitric Oxide Homeostasis in Salmonella typhimuriumPublished by Elsevier BV ,2008
- Spectropotentiometric and Structural Analysis of the Periplasmic Nitrate Reductase from Escherichia coliPublished by Elsevier BV ,2007
- Redox Fluctuations Frame Microbial Community Impacts on N-cycling Rates in a Humid Tropical Forest SoilBiogeochemistry, 2006
- NITROGEN CYCLING IN TROPICAL PLANTATION FORESTS: POTENTIAL CONTROLS ON NITROGEN RETENTIONEcological Applications, 2005
- Dissimilatory nitrate reduction to ammonium and responsible microorganisms in two Chinese and Australian paddy soilsSoil Biology and Biochemistry, 2002
- Structure and Spectroscopy of the Periplasmic Cytochrome c Nitrite Reductase from Escherichia coliBiochemistry, 2002
- Soil pH affects the processes reducing nitrate to nitrous oxide and di-nitrogenSoil Biology and Biochemistry, 1998
- Regulation of the hexaheme nitrite/nitric oxide reductase of Desulfovibrio desulfuricans, Wolinella succinogenes and Escherichia coliFEBS Letters, 1990
- Purification and further characterization of the second nitrate reductase of Escherichia coli K12JBIC Journal of Biological Inorganic Chemistry, 1990