Microtiter plate transformation using the LiAc/SS carrier DNA/PEG method
- 31 January 2007
- journal article
- Published by Springer Science and Business Media LLC in Nature Protocols
- Vol. 2 (1), 5-8
- https://doi.org/10.1038/nprot.2007.16
Abstract
Here, we describe a protocol that has been adapted for the transformation of yeast cells in 96-well microtiter plates. This protocol can be tailored for multiple applications and is suitable for high-throughput applications. It can be completed in 2-3 h, once the yeast cells have been grown depending on the heat shock used.Keywords
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