Commissural and intrinsic connections of the vestibular nuclei in the rabbit: a retrograde labeling study

Abstract

The intrinsic and commissural projection of the vestibular nuclei were investigated by means of retrograde transport of normal (HRP) and wheatgerm-agglutinated horseradish peroxidase (WGA-HRP). It was found that within each vestibular complex, the superior (SV), medial (MV) and descending (DV) vestibular nuclei are reciprocally connected. A rostrocaudally oriented column of medium-sized and large neurons, comprising the central SV and the magnocellular MV (MVmc) receives input from the surrounding neurons and does not reciprocate this projection. Efferents from group y terminate in the SV, MV and DV. The infracerebellar nucleus (INF) as well as the interstitial nucleus of the VIII the nerve (IN) supply fibers to the MV and DV. The neurons that participate in the commissural projection are distributed throughout the vestibular complex with the exception of the lateral vestibular nucleus (LV) and group x. The largest number of cells was found in the MV. The HRP labeled cells show a tendency to cluster into rostrocaudally oriented groups. Each nucleus projects to more than one contralateral nucleus. Group y shows a more extensive contralateral projection than the bordering INF. It was concluded that quantitative differences in connectivity were present between a core region in the vestibular complex and peripheral parts. This core region comprises the central SV, the LV, the MVmc and extends into the rostral DV. It receives predominantly intrinsic input from the surrounding vestibular neurons and is in contrast to these latter neurons only minimally involved in the commissural projection.