Prevalence of human papillomavirus types 16 and 18 in cervical adenocarcinoma and its precursors in Scottish patients
- 1 May 2006
- journal article
- research article
- Published by BMJ in International Journal of Gynecologic Cancer
- Vol. 16 (3), 1025-1031
- https://doi.org/10.1111/j.1525-1438.2006.00552.x
Abstract
Our aim was to determine the prevalence of human papillomavirus (HPV) types 16 and 18 in cervical adenocarcinoma (and its precursors) in Scottish patients. Nucleic acid was extracted from paraffin-embedded, formalin-fixed tissues. We examined 119 cases of invasive adenocarcinoma, 20 cases of adenocarcinoma in situ, and 16 cases of normal glandular epithelium. HPV DNA was detected by polymerase chain reaction using type-specific primers for the E6 and E7 genes of HPV-16 and HPV-18 with conformation of HPV genotype by subsequent restriction fragment length polymorphism. HPV DNA was identified in 87 (62.6%) cases, with HPV-16 being detectable in 65 (47%) cases and HPV-18 in 41 (29%) cases. All the cases of normal tissue tested negative for HPV-16 and/or HPV-18. No significant relation between infecting HPV type (16 or 18) and subtypes of disease (within the invasive category and between the preinvasive and the invasive categories) was noted. Our findings support that HPV-16, along with HPV-18, are likely to play a significant role in the pathogenesis of cervical adenocarcinomas and that cervical cancer screening strategies that incorporate oncogenic HPV testing, and prophylactic vaccines that target these types, will be beneficial for the reduction of adenocarcinoma and associated glandular precursors.Keywords
This publication has 29 references indexed in Scilit:
- The cervical cancer epidemic that screening has prevented in the UKThe Lancet, 2004
- American Cancer Society Guideline for the Early Detection of Cervical Neoplasia and CancerCA: A Cancer Journal for Clinicians, 2002
- Immunohistochemical Localization of Cdc6 in Squamous and Glandular Neoplasia of the Uterine CervixArchives Of Pathology & Laboratory Medicine, 2002
- Papillomaviruses and cancer: from basic studies to clinical applicationNature Reviews Cancer, 2002
- How predictive is a cervical smear suggesting glandular neoplasia?Cytopathology, 2002
- The role of human papillomavirus in cervical adenocarcinoma carcinogenesisEuropean Journal Of Cancer, 2001
- Differences in HPV 16- and HPV 18 E6/E7 oncogene expression between in situ and invasive adenocarcinomas of the cervix uteriVirchows Archiv, 2000
- The integration of HPV-18 DNA in cervical carcinomaMolecular Pathology, 1999
- Glandular Lesions of the Uterine CervixInternational Journal of Gynecological Pathology, 1997
- The human papillomavirus status of 114 endocervical adenocarcinoma cases by dot blot hybridizationHuman Pathology, 1993