Rapid Detection of Rifampin Resistance in Mycobacterium tuberculosis Isolates by Heteroduplex Analysis and Determination of Rifamycin Cross-Resistance in Rifampin-Resistant Isolates
Open Access
- 1 February 2003
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 41 (2), 816-818
- https://doi.org/10.1128/jcm.41.2.816-818.2003
Abstract
Direct heteroduplex analysis and a universal heteroduplex generator assay were performed to detect rifampin resistance rapidly in Turkish Mycobacterium tuberculosis isolates. Cross-resistance to rifapentine, rifabutin, and rifalazil was investigated. A relationship between specific mutations and resistance patterns, which can guide the choice of an appropriate therapeutic regimen for tuberculosis patients, was identified.Keywords
This publication has 9 references indexed in Scilit:
- Development of Rifapentine Susceptibility Tests for Mycobacterium tuberculosisAntimicrobial Agents and Chemotherapy, 1999
- Molecular genetic basis of antimicrobial agent resistance inMycobacterium tuberculosis: 1998 updateTubercle and Lung Disease, 1998
- Relationship between antimycobacterial activities of rifampicin, rifabutin and KRM-1648 and rpoB mutations of Mycobacterium tuberculosisJournal of Antimicrobial Chemotherapy, 1998
- Contribution of rpoB Mutations to Development of Rifamycin Cross-Resistance in Mycobacterium tuberculosisAntimicrobial Agents and Chemotherapy, 1998
- Evaluation of a Polymerase Chain Reaction–Based Universal Heteroduplex Generator Assay for Direct Detection of Rifampin Susceptibility ofMycobacterium tuberculosisfrom Sputum SpecimensClinical Infectious Diseases, 1998
- Genetic screening and testing by induced heteroduplex formationElectrophoresis, 1996
- Characterization of rifampin-resistance in pathogenic mycobacteriaAntimicrobial Agents and Chemotherapy, 1994
- Detection of rifampicin-resistance mutations in Mycobacterium tuberculosisThe Lancet, 1993
- Current methods of mutation detectionMutation Research, 1993