Variability of the Needle Essential Oils of Pinus heldreichii from Different Populations in Montenegro and Serbia
- 18 May 2007
- journal article
- research article
- Published by Wiley in Chemistry & Biodiversity
- Vol. 4 (5), 905-916
- https://doi.org/10.1002/cbdv.200790079
Abstract
The essential-oil compositions of Pinus heldreichii Christ. from Montenegro and Serbia are reported at the population level. Whitebark pine is a sub-endemic high-mountain Balkan pine relict of an anthropogenically reduced area, with large morphological diversity and insufficiently clear taxonomic position. In the pine-needle terpene profile from three populations from Montenegro, and one from Serbia, 101 compounds were detected, 72 of which could be identified (Table 3). The dominant constituents are limonene (26.3%), α-pinene (17.5%), germacrene D (13.5%), and β-caryophyllene (10.4%), comprising ca. 67.7% of the essential oil. Medium-to-high contents (0.5–10%) of the following 16 additional components were found: β-pinene, β-myrcene, α-humulene, δ-cadinene, α-muurolene, (E)-hex-2-enal, β-gurjunene, γ-muurolene, isopimarol, camphene, γ-cadinene, aromadendrene, β-bisabolene, trans-β-farnesene, α-cadinene, and (Z)-hex-3-en-1-ol. The similarity of the populations and the within-population variability was visualized by principle-component analysis (PCA) of eleven selected terpenes in 97 tree samples. Cluster and genetic analyses suggest closest connection between the two spatially most-distant populations I (Montenegro) and IV (Serbia). Based on the profile of the main sesquiterpene components, the studied populations from Montenegro and Serbia are more similar to the populations from Greece and the Central Balkan peninsula (Bosnia and Serbia–Kosovo) than to those on the furthest eastern margin of their natural range (Bulgaria).Keywords
This publication has 28 references indexed in Scilit:
- Chromosomal differentiation betweenPinus heldreichiiandPinus nigraAnnals of Forest Science, 2006
- Is there really more biodiversity in Mediterranean forest ecosystems?Taxon, 2005
- Origin identification of Pinus nigra populations in southwestern Europe using terpene composition variationsTrees, 2005
- Needle volatiles from five Pinus species growing in GreeceFlavour and Fragrance Journal, 2001
- Sesqui-, di-, and triterpenoids as chemosystematic markers in extant conifers—A reviewThe Botanical Review, 2001
- Phylogenetics ofPinus(Pinaceae) Based on Nuclear Ribosomal DNA Internal Transcribed Spacer Region SequencesMolecular Phylogenetics and Evolution, 1999
- Essential Oil ofPinus heldreichii, NeedlesJournal of Essential Oil Research, 1996
- Allozyme and chloroplast DNA variation in Italian and Greek populations of Pinus leucodermisHeredity, 1994
- Cembratrienols and other components of white bark pine (Pinus heldreichii) oleoresinPhytochemistry, 1994
- Serbian spruce, a narrow endemic, contains much genetic variationCanadian Journal of Forest Research, 1991