In Utero Ethanol Exposure Impairs Defenses Against ExperimentalGroup B Streptococcusin the Term Guinea Pig Lung
- 22 January 2009
- journal article
- Published by Wiley in Alcohol: Clinical and Experimental Research
- Vol. 33 (2), 300-306
- https://doi.org/10.1111/j.1530-0277.2008.00833.x
Abstract
Background: The effects of fetal alcohol exposure on the risks of neonatal lung injury and infection remain under investigation. The resident alveolar macrophage (AM) is the first line of immune defense against pulmonary infections. In utero ethanol (ETOH) exposure deranges the function of both premature and term guinea pig AM. We hypothesized that fetal ETOH exposure would increase the risk of pulmonary infection in vivo. Methods: We developed a novel in vivo model of group B Streptococcus (GBS) pneumonia using our established guinea pig model of fetal ETOH exposure. Timed‐pregnant guinea pigs were pair fed ±ETOH and some were supplemented with the glutathione (GSH) precursor S‐adenosyl‐methionine (SAM‐e). Term pups were given GBS intratracheally while some were pretreated with inhaled GSH prior to the experimental GBS. Neonatal lung and whole blood were evaluated for GBS while isolated AM were evaluated using fluorescent microscopy for GBS phagocytosis. Results: Ethanol‐exposed pups demonstrated increased lung infection and sepsis while AM phagocytosis of GBS was deficient compared with control. When SAM‐e was added to the maternal diet containing ETOH, neonatal lung and systemic infection from GBS was attenuated and AM phagocytosis was improved. Inhaled GSH therapy prior to GBS similarly protected the ETOH‐exposed pup from lung and systemic infection. Conclusions: In utero ETOH exposure impaired the neonatal lung’s defense against experimental GBS, while maintaining GSH availability protected the ETOH‐exposed lung. This study suggested that fetal alcohol exposure deranges the neonatal lung’s defense against bacterial infection, and support further investigations into the potential therapeutic role for exogenous GSH to augment neonatal AM function.Keywords
This publication has 66 references indexed in Scilit:
- Chronic Alcoholism Alters Systemic and Pulmonary Glutathione Redox StatusAmerican Journal of Respiratory and Critical Care Medicine, 2007
- GM-CSF receptor expression and signaling is decreased in lungs of ethanol-fed ratsAmerican Journal of Physiology-Lung Cellular and Molecular Physiology, 2006
- Acute and Chronic Alcohol Abuse Modulate ImmunityAlcohol: Clinical and Experimental Research, 2006
- Early-Onset Group B Streptococcal Disease in the Era of Maternal ScreeningPublished by American Academy of Pediatrics (AAP) ,2005
- Role for Cystic Fibrosis Transmembrane Conductance Regulator Protein in a Glutathione Response to Bronchopulmonary Pseudomonas InfectionInfection and Immunity, 2004
- Alcohol Consumption during Pregnancy and the Risk of Preterm DeliveryAmerican Journal of Epidemiology, 2004
- Effects of In Utero Alcohol Exposure on B-Cell Development in the Murine Fetal LiverAlcohol: Clinical and Experimental Research, 1998
- Influence of Ethanol Consumption on Immune Competence of Adult Animals Exposed to Ethanol In UteroAlcohol: Clinical and Experimental Research, 1998
- The Effect of Cold Stress on Lymphocyte Proliferation in Fetal Ethanol‐Exposed RatsAlcohol: Clinical and Experimental Research, 1997
- Developmental aspects of pulmonary defenses in childrenPediatric Pulmonology, 1995