Phytochemical Profile of Main Antioxidants in Different Fractions of Purple and Blue Wheat, and Black Barley
- 26 September 2007
- journal article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 55 (21), 8541-8547
- https://doi.org/10.1021/jf072021j
Abstract
Two pigmented wheat genotypes (blue and purple) and two black barley genotypes were fractionated in bran and flour fractions, examined, and compared for their free radical scavenging properties against 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical cation (Trolox equivalent antioxidant capacity, TEAC), ferric reducing antioxidant power (FRAP), total phenolic content (TPC), phenolic acid composition, carotenoid composition, and total anthocyanin content. The results showed that fractionation has a significant influence on the antioxidant properties, TPC, anthocyanin and carotenoid contents, and phenolic acid composition. Bran fractions had the greatest antioxidant activities (1.9–2.3 mmol TEAC/100 g) in all four grain genotypes and were 3–5-fold higher than the respective flour fractions (0.4–0.7 mmol TEAC/100 g). Ferulic acid was the predominant phenolic acid in wheat genotypes (bran fractions) while p-coumaric acid was the predominant phenolic acid in the bran fractions of barley genotypes. High-performance liquid chromatography analysis detected the presence of lutein and zeaxanthin in all fractions with different distribution patterns within the genotypes. The highest contents of anthocyanins were found in the middlings of black barley genotypes or in the shorts of blue and purple wheat. These data suggest the possibility to improve the antioxidant release from cereal-based food through selection of postharvest treatments.This publication has 40 references indexed in Scilit:
- Contents of Phenolic Acids, Alkyl- and Alkenylresorcinols, and Avenanthramides in Commercial Grain ProductsJournal of Agricultural and Food Chemistry, 2005
- Carotenoid, Tocopherol, Phenolic Acid, and Antioxidant Properties of Maryland-Grown Soft WheatJournal of Agricultural and Food Chemistry, 2005
- Phenolic Acid, Tocopherol and Carotenoid Compositions, and Antioxidant Functions of Hard Red Winter Wheat BranJournal of Agricultural and Food Chemistry, 2005
- Comparison of Swiss Red Wheat Grain and Fractions for Their Antioxidant PropertiesJournal of Agricultural and Food Chemistry, 2004
- Absorption of Hydroxycinnamates in Humans after High-Bran Cereal ConsumptionJournal of Agricultural and Food Chemistry, 2003
- Composition and Stability of Anthocyanins in Blue-Grained WheatJournal of Agricultural and Food Chemistry, 2003
- Application of the 2,2‘-Azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Radical Cation Assay to a Flow Injection System for the Evaluation of Antioxidant Activity of Some Pure Compounds and BeveragesJournal of Agricultural and Food Chemistry, 2002
- A Rapid Method for Quantifying Total Anthocyanins in Blue Aleurone and Purple Pericarp WheatsCereal Chemistry Journal, 1999
- Variation in Ferulic Acid Concentration among Diverse Barley Cultivars Measured by HPLC and MicrospectrophotometryJournal of Agricultural and Food Chemistry, 1998
- Free, esterified, and insoluble-bound phenolic acids. 3. Composition of phenolic acids in cereal and potato floursJournal of Agricultural and Food Chemistry, 1982