A comparative analysis of streaming potentials in vivo and in vitro
- 1 September 1992
- journal article
- research article
- Published by Wiley in Journal of Orthopaedic Research
- Vol. 10 (5), 710-719
- https://doi.org/10.1002/jor.1100100513
Abstract
Streaming potentials (SPs) measured in vivo at a specific site on intact cortical bone (canine tibia) have been compared with measurements from the same site in vitro, tested as an excised bone strip soaked in Hank's balanced salt solution. The amplitude of SPs per periosteal strain in vitro was larger in 13 tibias than in vivo (by an average X6.5 at 1 Hz), but values per transcortical strain difference were similar. In vitro, SP magnitudes rose more sharply to an asymptotic value as a function of bending frequency than did in vivo signals, possibly because of a difference in the internal state of canaliculi and/or Haversian systems. Similarly, SP response to step‐loading decreased to zero more slowly with time in vitro than in vivo. Difficulties encountered in preliminary measurements due to electrical shunting through electrolyte and soft tissues suggest the need for caution in using both in vivo and in vitro SP measurements to extrapolate to electric field strengths on the cellular level.Keywords
This publication has 33 references indexed in Scilit:
- Mechanoelectric transduction in boneJournal of Materials Research, 1989
- Neutron diffraction studies of collagen in fully mineralized boneJournal of Molecular Biology, 1985
- Endogenous Ionic Currents Traverse Intact and Damaged BoneScience, 1984
- Streaming potential and the electromechanical response of physiologically-moist boneJournal of Biomechanics, 1982
- Midroelectrode studies of stress‐generated potentials in four‐point bending of boneJournal of Biomedical Materials Research, 1979
- Microelectrode study of stress-generated potentials obtained from uniform and nonuniform compression of human boneJournal of Biomedical Materials Research, 1979
- Strain related electrical potentials recorded in vitro and in vivoCalcified Tissue International, 1977
- Effect of fibrillar structure of pericanalicular and intercanalicular bone on X-ray absorptionThe Anatomical Record, 1970