Sialylation of Campylobacter jejuni Lipo-Oligosaccharides: Impact on Phagocytosis and Cytokine Production in Mice
Open Access
- 28 March 2012
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 7 (3), e34416
- https://doi.org/10.1371/journal.pone.0034416
Abstract
Guillain-Barré syndrome (GBS) is a post-infectious polyradiculoneuropathy, frequently associated with antecedent Campylobacter jejuni (C. jejuni) infection. The presence of sialic acid on C. jejuni lipo-oligosaccharide (LOS) is considered a risk factor for development of GBS as it crucially determines the structural homology between LOS and gangliosides, explaining the induction of cross-reactive neurotoxic antibodies. Sialylated C. jejuni are recognised by TLR4 and sialoadhesin; however, the functional implications of these interactions in vivo are unknown. In this study we investigated the effects of bacterial sialylation on phagocytosis and cytokine secretion by mouse myeloid cells in vitro and in vivo. Using fluorescently labelled GM1a/GD1a ganglioside-mimicking C. jejuni strains and corresponding (Cst-II-mutant) control strains lacking sialic acid, we show that sialylated C. jejuni was more efficiently phagocytosed in vitro by BM-MΦ, but not by BM-DC. In addition, LOS sialylation increased the production of IL-10, IL-6 and IFN-β by both BM-MΦ and BM-DC. Subsequent in vivo experiments revealed that sialylation augmented the deposition of fluorescent bacteria in splenic DC, but not macrophages. In addition, sialylation significantly amplified the production of type I interferons, which was independent of pDC. These results identify novel immune stimulatory effects of C. jejuni sialylation, which may be important in inducing cross-reactive humoral responses that cause GBS.This publication has 34 references indexed in Scilit:
- Campylobacter jejuni Lipooligosaccharides Modulate Dendritic Cell-Mediated T Cell Polarization in a Sialic Acid Linkage-Dependent MannerInfection and Immunity, 2011
- Characterization of the Specific Interaction between Sialoadhesin and Sialylated Campylobacter jejuni LipooligosaccharidesInfection and Immunity, 2010
- Type I IFN enhances follicular B cell contribution to the T cell–independent antibody responseThe Journal of Experimental Medicine, 2010
- Activation of Human and Chicken Toll-Like Receptors by Campylobacter sppInfection and Immunity, 2010
- Campylobacter jejuni -Induced Activation of Dendritic Cells Involves Cooperative Signaling through Toll-Like Receptor 4 (TLR4)-MyD88 and TLR4-TRIF AxesInfection and Immunity, 2009
- Targeting to porcine sialoadhesin receptor receptor improves antigen presentation to T cellsVeterinary Research, 2008
- The Sialylated Lipooligosaccharide Outer Core in Campylobacter jejuni Is an Important Determinant for Epithelial Cell InvasionInfection and Immunity, 2008
- Host-Pathogen Interactions inCampylobacterInfections: the Host PerspectiveClinical Microbiology Reviews, 2008
- Siglecs and their roles in the immune systemNature Reviews Immunology, 2007
- Structural Characterization of Campylobacter jejuni Lipooligosaccharide Outer Cores Associated with Guillain-Barré and Miller Fisher SyndromesInfection and Immunity, 2007