Synthesis of Molecularly Imprinted Polymer Nanoparticles for α-Casein Detection Using Surface Plasmon Resonance as a Milk Allergen Sensor
- 26 January 2018
- journal article
- research article
- Published by American Chemical Society (ACS) in ACS Sensors
- Vol. 3 (2), 418-424
- https://doi.org/10.1021/acssensors.7b00850
Abstract
Food recalls due to undeclared allergens or contamination are costly to the food manufacturing industry worldwide. As the industry strives for better manufacturing efficiencies over a diverse range of food products, there is a need for the development of new analytical techniques to improve monitoring of the presence of unintended food allergens during the food manufacturing process. In particular, the monitoring of wash samples from cleaning in place systems (CIP), used in the cleaning of food processing equipment, would allow for the effective removal of allergen containing ingredients in between food batches. Casein proteins constitute the biggest group of proteins in milk and hence are the most common milk protein allergen in food ingredients. As such, these proteins could present an ideal analyte for cleaning validation. In this work, molecularly imprinted polymer nanoparticles (nanoMIPs) with high affinity toward bovine α-casein were synthesized using a solid-phase imprinting method. The nanoMIPs were then characterized and incorporated into label free surface plasmon resonance (SPR) based sensor. The nanoMIPs demonstrated good binding affinity and selectivity toward α-casein (KD ∼ 10 × 10–9 M). This simple affinity sensor demonstrated the quantitative detection of α-casein achieving a detection limit of 127 ± 97.6 ng mL–1 (0.127 ppm) which is far superior to existing commercially available ELISA kits. Recoveries from spiked CIP wastewater samples were within the acceptable range (87–120%). The reported sensor could allow food manufacturers to adequately monitor and manage food allergen risk in food processing environments while ensuring that the food produced is safe for the consumer.Keywords
Funding Information
- Innovate UK (34312-241239)
This publication has 32 references indexed in Scilit:
- Computational Design of Peptide Ligands for Ochratoxin AToxins, 2013
- Mass spectrometry detection of egg proteins in red wines treated with egg whiteFood Control, 2012
- Simultaneous, quantitative detection of five whey proteins in multiple samples by surface plasmon resonanceInternational Dairy Journal, 2010
- The Key Events Dose-Response Framework: A Foundation for Examining Variability in Elicitation Thresholds for Food AllergensCritical Reviews in Food Science and Nutrition, 2009
- Commercialized rapid immunoanalytical tests for determination of allergenic food proteins: an overviewAnalytical and Bioanalytical Chemistry, 2009
- Peptide Imprinted Polymer Nanoparticles: A Plastic AntibodyJournal of the American Chemical Society, 2008
- Specific and Sensitive Enzyme-Linked Immunosorbent Assays for Analysis of Residual Allergenic Food Proteins in Commercial Bottled Wine Fined with Egg White, Milk, and Nongrape-Derived TanninsJournal of Agricultural and Food Chemistry, 2007
- A ‘gold cluster-linked immunosorbent assay’: Optical near-field biosensor chip for the detection of allergenic β-lactoglobulin in processed milk matricesJournal of Biotechnology, 2007
- A localized surface plasmon resonance based immunosensor for the detection of casein in milkScience and Technology of Advanced Materials, 2007
- Analysis and Evaluation of Food Manufacturing Practices Used to Address Allergen ConcernsComprehensive Reviews in Food Science and Food Safety, 2006