The 56 kDa protein of human genital skin fibroblasts is identical to that radiolabelled by [3H]dihydrotestosterone 17β-bromoacetate
- 1 September 1989
- journal article
- research article
- Published by Elsevier BV in The Journal of Steroid Biochemistry and Molecular Biology
- Vol. 33 (3), 389-394
- https://doi.org/10.1016/0022-4731(89)90328-2
Abstract
Analysis of soluble proteins from human genital skin fibroblasts by two-dimensional polyacrylamide gel electrophoresis reveals an abundant protein doublet of mol. wt 56,000 with isoelectric points (pI) of 6.7 and 6.5. This protein is absent in non-genital skin fibroblasts as well as in genital skin fibroblasts of most patients with complete forms of androgen insensitivity. The protein specifically binds androgen. A protein of similar estimated molecular weight (58,000) from human genital skin fibroblasts has recently been found to be covalently radiolabelled by the affinity ligand dihydrotestosterone 17β-bromoacetate (DHT-BA). In the present study these proteins have been found to be indistinguishable on one- and two-dimensional gel electrophoresis. Antibodies raised against the 56 kDa pI 6.7/6.5 protein also recognized the protein covalently radiolabelled by DHT-BA. A third protein of estimated mol. wt 59,000 has been found to be associated with several steroid hormone receptor complexes but has no known ligand binding activity. This protein was found to be clearly separable from the 56/58 kDa protein on two-dimensional gel electrophoresis as it has a more acidic pI of approximately 5.4. Furthermore, antibodies against the 59 kDa protein do not recognize the 56 kDa species, and vice versa.This publication has 21 references indexed in Scilit:
- Biochemical Characterization of the Protein Affinity Labeled by Dihydrotestosterone 17β-Bromoacetate: Comparison with the Human Androgen Receptor*Endocrinology, 1989
- An abundant 56 kD protein with low affinity androgen binding: Another member of the steroid/thyroid receptor family?Biochemical and Biophysical Research Communications, 1988
- High efficiency covalent radiolabeling of the human androgen receptor. Studies in cultured fibroblasts using dihydrotestosterone 17 beta-bromoacetate.JCI Insight, 1988
- A 59-kilodalton protein associated with progestin, estrogen, androgen, and glucocorticoid receptorsBiochemistry, 1986
- Affinity labeling of the androgen receptor in rat prostate cytosol with 17.beta.-[(bromoacetyl)oxy]-5.alpha.-androstan-3-oneBiochemistry, 1984
- 56K fibroblast protein not specific for Duchenne muscular dystrophy but for skin biopsy siteNature, 1983
- Purification and characterization of androgen receptor from steer seminal vesicleBiochemistry, 1982
- Analysis of fibroblast proteins from patients with Duchenne muscular dystrophy by two-dimensional gel electrophoresisNature, 1982
- Androgen receptor in human skin fibroblasts characterization of a specific 17β-hydroxy-5α-androstan-3-one-protein complex in cell sonicates and nucleiSteroids, 1975
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970