Comparison of phosphatidylinositol‐3‐kinase signalling within a panel of human colorectal cancer cell lines with mutant or wild‐type PIK3CA
Open Access
- 24 August 2005
- journal article
- Published by Wiley in FEBS Letters
- Vol. 579 (23), 5123-5128
- https://doi.org/10.1016/j.febslet.2005.07.096
Abstract
Recent studies have identified conserved missense mutations in PIK3CA, the gene encoding the catalytic phosphatidylinositol‐3‐kinase subunit p110α, in a variety of human cancers. Further investigation demonstrated that PIK3CA mutations lead to increased basal phosphatidylinositol‐3‐kinase activity, promoting cell growth and invasion [Samuels, Y., Diaz, L.A., Jr., Schmidt‐Kittler, O., Cummins, J.M., Delong, L., Cheong, I., Rago, C., Huso, D.L., Lengauer, C., Kinzler, K.W., Vogelstein, B. and Velculescu, V.E. (2005) Mutant PIK3CA promotes cell growth and invasion of human cancer cells. Cancer Cell 7, 561–573]. A panel of commonly used colorectal cancer cell lines was screened for these PIK3CA mutations. Constitutive and IGF‐1‐stimulated phosphatidylinositol‐3‐kinase activity, signal response and duration were assessed. In the assays used no differences distinguished cells carrying PIK3CA mutations indicating that these mutations did not significantly alter growth factor stimulated or steady state phosphatidylinositol‐3‐kinase activity in normal cell culture conditions.Keywords
This publication has 13 references indexed in Scilit:
- Mutant PIK3CA promotes cell growth and invasion of human cancer cellsCancer Cell, 2005
- Functional Analysis of PIK3CA Gene Mutations in Human Colorectal CancerCancer Research, 2005
- Construction and characterization of multiple human colon cancer cell lines for inducibly regulated gene expressionJournal of Cellular Biochemistry, 2005
- Mutations of PIK3CA in Anaplastic Oligodendrogliomas, High-Grade Astrocytomas, and MedulloblastomasCancer Research, 2004
- Muscarinic-receptor-mediated inhibition of insulin-like growth factor-1 receptor-stimulated phosphoinositide 3-kinase signalling in 1321N1 astrocytoma cellsBiochemical Journal, 2004
- High Frequency of Mutations of the PIK3CA Gene in Human CancersScience, 2004
- PI3K/Akt signalling pathway and cancerCancer Treatment Reviews, 2004
- The phosphatidylinositol 3-Kinase–AKT pathway in human cancerNature Reviews Cancer, 2002
- The Phosphoinositide 3-Kinase PathwayScience, 2002
- A genetic model for colorectal tumorigenesisCell, 1990