Roles of zinc-fingers and homeoboxes 1 during the proliferation, migration, and invasion of glioblastoma cells
Open Access
- 28 March 2017
- journal article
- research article
- Published by IOS Press in Tumor Biology
- Vol. 39 (3)
- https://doi.org/10.1177/1010428317694575
Abstract
Zinc-fingers and homeoboxes 1 (ZHX1) is a nuclear transcription repressor and known to be involved in cell differentiation and tumorigenesis. However, the pathophysiological roles of ZHX1 have not been characterized in glioblastoma. We examined ZHX1 expression in glioblastoma patients’ tissues and analyzed overall survival of the patients based on expression level of ZHX1. We also examined the effects of ZHX1 on proliferation and motility of glioblastoma cells. In silico analysis and immunohistochemical studies showed that the messenger RNA and protein expressions of ZHX1 were higher in the tissues of glioblastoma patients than in normal brain tissues, and that its overexpression was associated with reduced survival. In vitro, the downregulation of ZHX1 decreased the proliferation, migration, and invasion of glioblastoma cells, whereas its upregulation had the opposite effects. In addition, we showed ZHX1 could contribute to glioblastoma progression via the regulations of TWIST1 and SNAI2. Taken together, this study demonstrates that ZHX1 plays crucial roles in the progression of glioblastoma, and its findings suggest that ZHX1 be viewed as a potential prognostic maker and therapeutic target of glioblastoma.Keywords
This publication has 35 references indexed in Scilit:
- Current Challenges in Glioblastoma: Intratumour Heterogeneity, Residual Disease, and Models to Predict Disease RecurrenceFrontiers in Oncology, 2015
- Zinc fingers and homeoboxes family in human diseasesCancer Gene Therapy, 2015
- Expression and roles of Wilms' tumor 1‐associating protein in glioblastomaCancer Science, 2012
- BS69, a corepressor interacting with ZHX1, is a bifunctional transcription factorFrontiers in Bioscience-Landmark, 2007
- Radiotherapy plus Concomitant and Adjuvant Temozolomide for GlioblastomaThe New England Journal of Medicine, 2005
- Zinc-fingers and homeoboxes (ZHX) 2, a novel member of the ZHX family, functions as a transcriptional repressorBiochemical Journal, 2003
- Analysis of zinc-fingers and homeoboxes (ZHX)-1-interacting proteins: molecular cloning and characterization of a member of the ZHX family, ZHX3Biochemical Journal, 2003
- Functional analysis and the molecular dissection of zinc-fingers and homeoboxes 1 (ZHX1)Biochemical and Biophysical Research Communications, 2002
- Rat zinc-fingers and homeoboxes 1 (ZHX1), a nuclear factor-YA-interacting nuclear protein, forms a homodimerGene, 2002
- zhx-1:A Novel Mouse Homeodomain Protein Containing Two Zinc-Fingers and Five HomeodomainsBiochemical and Biophysical Research Communications, 1996