Specialized DNA Arrays for the Differentiation of Pancreatic Tumors
- 15 November 2005
- journal article
- Published by American Association for Cancer Research (AACR) in Clinical Cancer Research
- Vol. 11 (22), 8048-8054
- https://doi.org/10.1158/1078-0432.ccr-05-1274
Abstract
Purpose: Malignant tumors of the pancreas are frequently indistinguishable from inflammatory tumors arising in the context of a chronic pancreatitis with the use of conventional imaging techniques. Thus, cytologic analysis of cells obtained by abdominal ultrasound, computed tomography, or endoscopic ultrasound–guided fine needle aspiration biopsy is required for diagnosis. However, the reliability of cytologic analyses of pancreatic fine needle aspirates remains unsatisfactory, with a diagnostic accuracy of ≤80%. The purpose of the current study was therefore to develop a novel diagnostic approach based on expression profiling of biopsy material using a specialized diagnostic cDNA array. Experimental Design: Previous gene expression profiling studies were reevaluated to design a 558-feature diagnostic array. Minimal amounts of residual material from pancreatic cytology samples as well as surgically resected tumor and control tissue specimens were analyzed using the diagnostic array and a newly developed statistical classification system. Results and Conclusions: Our diagnostic approach resulted in 95% accurate differentiation between ductal adenocarcinomas and nonmalignant tumors of the pancreas. The diagnostic array, in conjunction with conventional diagnostic procedures, is thus suitable to significantly improve the reliability of pancreatic cancer diagnostics and can be expected to become a valuable new tool in the routine workup of suspect masses in the pancreas.Keywords
This publication has 23 references indexed in Scilit:
- Genome-wide cDNA microarray analysis of gene expression profiles in pancreatic cancers using populations of tumor cells and normal ductal epithelial cells selected for purity by laser microdissectionOncogene, 2004
- MUC1 and MUC2 expression in pancreatic ductal carcinoma obtained by fine-needle aspirationCancer, 2003
- Endoscopic Ultrasound–Guided Fine Needle Aspiration of the PancreasActa Cytologica, 2003
- Expression profiling of microdissected pancreatic adenocarcinomasOncogene, 2002
- Endoscopic ultrasound-guided fine-needle aspiration biopsyCancer, 2002
- Gene expression profiling predicts clinical outcome of breast cancerNature, 2002
- Prospective evaluation of the contribution of K-ras mutational analysis and CA 19.9 measurement to cytological diagnosis in patients with clinical suspicion of pancreatic cancerEuropean Journal Of Cancer, 2000
- Value of endoscopic ultrasound guided fine needle aspiration biopsy in the diagnosis of solid pancreatic massesGut, 2000
- How to Report Statistics in MedicineThe Nurse Practitioner, 1997
- Ki-ras Mutations and the Carcinoembryonic Antigen Level in Fine Needle Aspirates of the PancreasActa Cytologica, 1997