A Rapid and Simple Procedure for Purification of Indole-3-Acetic Acid Prior to GC-SIM-MS Analysis
- 1 March 1988
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 86 (3), 822-825
- https://doi.org/10.1104/pp.86.3.822
Abstract
A simple and rapid procedure for the purification of indole-3-acetic acid prior to gas chromatography-selected ion monitoring-mass spectrographic analysis was developed using an amino anion exchange minicolumn and a short high resolution C18 column. Since multiple samples can be prepared at one time, the procedure is more rapid and the sample preparation time is reduced to one-third that normally required. In addition, the final recovery was improved by 40 to 50% over that of a solvent partitioning procedure.Keywords
This publication has 10 references indexed in Scilit:
- Measurement of Indole-3-Acetic Acid in Peach Fruits (Prunus persica L. Batsch cv Redhaven) during DevelopmentPlant Physiology, 1987
- Investigations on the Nature of the Auxin-Wave in the Cambial Region of Pine StemsPlant Physiology, 1987
- 13C6-[Benzene Ring]-Indole-3-Acetic AcidPlant Physiology, 1986
- Endogenous Indole-3-Acetic Acid in the Stem of Tobacco in Relation to Flower Neoformation as Measured by Mass Spectroscopic AssayPlant Physiology, 1984
- Effect of Deseeding on the Indole-3-acetic Acid Content of Shoots and Roots of Zea mays SeedlingsPlant Physiology, 1983
- Gravitational effects on plant growth hormone concentrationAdvances in Space Research, 1983
- Distribution of Free and Ester Indole-3-Acetic Acid in the Cortex and Stele of the Zea mays MesocotylPlant Physiology, 1982
- Double-standard isotope dilution assayAnalytical Biochemistry, 1981
- Synthesis of 4,5,6,7 and 2,4,5,6,7 Deuterium-labeled Indole-3-Acetic Acid for Use in Mass Spectrometric AssaysPlant Physiology, 1980
- Isolation of indole-3-acetic acid from corn kernels & etiolated corn seedlingsPlant Physiology, 1961