Androgenic responses of 64 ornamental pepper (Capsicum annuum L.)genotypes to shed-microspore culture in the autumn season
- 1 January 2016
- journal article
- research article
- Published by The Scientific and Technological Research Council of Turkey (TUBITAK-ULAKBIM) - DIGITAL COMMONS JOURNALS in TURKISH JOURNAL OF BIOLOGY
- Vol. 40 (3), 706-717
- https://doi.org/10.3906/biy-1505-41
Abstract
The recent gradual increase in interest and demand for multipurpose ornamental peppers has encouraged intensive breeding studies, especially for use in landscape. However, information in the literature about haploidy is limited. Thus, first, we intend to identify proper morphological markers for quick selection of suitable flower buds containing convenient microspores in two sample ornamental pepper genotypes with and without anthocyanin in external appearance. Secondly, the androgenic responses of 64 genotypes are determined in the autumn season through shed-microspore culture. Based on DAPI-assisted results, the calyx/bud ratio can be used as an effective morphological marker for proper bud selection in both full anthocyanin (70%-80%) and green-looking (70%-85%) genotypes. The indicator of anthocyanin pigmentation in anthers, as used in peppers, is a convenient marker for green-looking genotypes (10%-40%), although it is not suitable for genotypes with full anthocyanin (70%-90%). Of a total of 64 genotypes, 48 responded to the shed-microspore culture and produced embryos in varying ratios. The highest average yields of total and normal-looking embryos per flower bud were found to be 12.7 and 1.48, respectively. The promising embryo formation results obtained in autumn verified that the shed-microspore culture technique can be used successfully in different ornamental pepper genotypes with landscape value for double haploid plant production.Keywords
This publication has 18 references indexed in Scilit:
- In vitro androgenic response of minipaprika (Capsicum annuum L.) genotypes in different culture mediaHorticulture, Environment, and Biotechnology, 2013
- Morphological markers to correlate bud and anther development with microsporogenesis and microgametogenesis in pepper (Capsicum annuum L.)Acta Physiologiae Plantarum, 2012
- Induction of microspore-derived embryos by anther culture in selected pepper genotypesAFRICAN JOURNAL OF BIOTECHNOLOGY, 2011
- Refinement of shed-microspore culture protocol to increase normal embryos production in hot pepper (Capsicum annuum L.)Scientia Horticulturae, 2011
- Individual reaction of Capsicum F2 hybrid genotypes in anther culturesEuphytica, 2009
- The effect of liquid and solid medium on production of winter triticale (×TriticosecaleWittm.) anther-derived embryos and plantsCereal Research Communications, 2007
- Thidiazuron induced somatic embryogenesis and plant regeneration in Capsicum annuumBiologia plantarum, 2006
- Influence of growing season and donor plant age on anther culture response of some pepper cultivars (Capsicum annuum L.)Scientia Horticulturae, 2006
- Evaluation of crucial factors for implementing shed-microspore culture of Indonesian hot pepper (Capsicum annuum L.) cultivarsScientia Horticulturae, 2006
- Pepper (capsicum annuum L.) regenerants obtained by direct somatic embryogenesis fail to develop a shootIn Vitro Cellular & Developmental Biology - Plant, 2003