Circulating CD10−/CD16low neutrophils provide a quantitative index of active bone marrow neutrophil release
- 31 October 2005
- journal article
- Published by Wiley in British Journal of Haematology
- Vol. 131 (4), 508-519
- https://doi.org/10.1111/j.1365-2141.2005.05794.x
Abstract
Circulating neutrophil phenotype and function are altered during neutrophilia associated with acute inflammatory states, however, the contribution of bone marrow neutrophil release to these changes has been difficult to quantify in humans. Accelerated release of neutrophils, with potentially distinct attributes, from the bone marrow and their dilution within the circulating pool may produce these apparent changes. Unfortunately selective analysis of these newly emergent neutrophils is difficult given their morphologic similarity to those already in the circulation and the coincident effect of soluble inflammatory mediators on circulating neutrophil phenotype and function. Using whole blood flow cytometry and cardiac surgery as an inflammatory stimulus, we demonstrate the emergence of a unique subpopulation of circulating neutrophils characterised as CD10(-)/CD16(low), indicative of active bone marrow neutrophil release peri-operatively. CD10(-)/CD16(low) neutrophils emerge at the same operative stages as band forms and a left shift, yet represent over 40% of circulating neutrophils postoperatively, and generate a greater stimulus-induced [Ca(2+)](i) flux than their CD10(+) counterparts. We conclude that CD10(-)/CD16(low) neutrophils represent a significant proportion of the circulating pool after cardiac surgery and that bone marrow release, a major contributor to neutrophilia, influences the phenotype and functional activity of circulating neutrophils following this acute inflammatory stimulus.Keywords
This publication has 42 references indexed in Scilit:
- Reduced Neutrophil CD10 Expression in Nonhuman Primates and Humans After In Vivo Challenge with E. coli or LipopolysaccharideShock, 2003
- Phagosomal oxidative activity during β2 integrin (CR3)-mediated phagocytosis by neutrophils is triggered by a non-restricted Ca2+signal: Ca2+ controls time not spaceJournal of Cell Science, 2003
- Expression Patterns of the Lipopolysaccharide Receptor CD14, and the FC?? Receptors CD16 and CD64 on Polymorphonuclear Neutrophils: Data from Patients with Severe Bacterial Infections and Lipopolysaccharide-Exposed CellsShock, 2003
- Ethylenediaminetetraacetic acid plus citrate-theophylline-adenosine-dipyridamole (EDTA-CTAD): A novel anticoagulant for the flow cytometric assessment of platelet and neutrophil activation ex vivo in whole bloodCytometry, 2002
- Role of the neutrophil in the development of systemic inflammatory response syndrome and sepsis following abdominal aortic surgeryBritish Journal of Surgery, 2001
- Neutral endopeptidase modulation of septic shock.The Journal of Experimental Medicine, 1995
- Neutrophil Mac-1 and MEL-14 Adhesion Proteins Inversely Regulated by Chemotactic FactorsScience, 1989
- The Pi-linked receptor FcRIII is released on stimulation of neutrophilsNature, 1988
- Extra- and intracellular Ca2+ requirements for lysosomal enzyme secretion in human neutrophilsInflammation Research, 1982
- Neutrophil Kinetics in Acute Infection*JCI Insight, 1967