Interference by Anti-Cancer Chemotherapeutic Agents in the MTT-Tumor Chemosensitivity Assay
- 1 April 2004
- journal article
- Published by S. Karger AG in Chemotherapy
- Vol. 50 (1), 43-50
- https://doi.org/10.1159/000077285
Abstract
Background: One of the major goals of oncology is to predict the response of patients with cancer to chemotherapeutic agents by employing laboratory methods variously called ‘tumor chemosensitivity assays’, ‘drug response assays’, or ‘drug sensitivity assays’, in vitro. The MTT assay is one of the methods used to predict the drug response in malignancies. However, it may suffer from interference by the anticancer drugs with the MTT assay. Methods: The MTT assay, a colorimetric viability assay, was checked in a cell-free system in terms of its possible chemical interactions with 22 different anticancer drugs. Results: It was found that epirubicine, paclitaxel, doxetaxel, and cisplatin caused a relatively significant increase in absorbance values, resulting in the MTT assay giving rise to false results (untrue increase in viability) although most of the drugs tested did not seem to cause any significant change. Conclusion: It was concluded that before employing the MTT assay, drugs (or any kind of substances) to be included in the assay should be checked first in terms of possible chemical interactions with MTT, otherwise it may be impossible to evaluate the MTT viability assay results correctly.Keywords
This publication has 13 references indexed in Scilit:
- Use of an ATP-based chemosensitivity assay to design new combinations of high-concentration doxorubicin with other drugs for recurrent ovarian cancerAnti-Cancer Drugs, 2002
- DNA strand breaks and cell cycle perturbation in Herceptin treated breast cancer cell lines*Breast Cancer Research and Treatment, 2001
- Towards in-vitro prediction of an in-vivo cytostatic response of human tumor cells with a fast chemosensitivity assayToxicology, 2001
- Interaction between novel anticancer agents and radiation in non-small cell lung cancer cell linesLung Cancer, 2001
- An evaluation of the colorimetric assays based on enzymatic reactions used in the measurement of human natural cytotoxicityJournal of Immunological Methods, 2001
- In vitro topo II–DNA complex accumulation and cytotoxicity of etoposide in leukaemic cells from patients with acute myelogenous and chronic lymphocytic leukaemiaLeukemia Research, 2001
- 4-(N-Hydroxyphenyl)retinamide Can Selectively Induce Apoptosis in Human Epidermoid Carcinoma Cells But Not in Normal Dermal FibroblastsCancer Investigation, 2001
- Comparison of the LDH and MTT assays for quantifying cell death: validity for neuronal apoptosis?Journal of Neuroscience Methods, 2000
- P-Glycoprotein-Expressing Tumor Cells Are Resistant to Anticancer Drugs in Human Gastrointestinal CancerSurgery Today, 1999
- Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assaysJournal of Immunological Methods, 1983