Reconstitution of protein targeting to the inner envelope membrane of chloroplasts
Open Access
- 23 October 2006
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 175 (2), 249-259
- https://doi.org/10.1083/jcb.200605162
Abstract
The chloroplast envelope plays critical roles in the synthesis and regulated transport of key metabolites, including intermediates in photosynthesis and lipid metabolism. Despite this importance, the biogenesis of the envelope membranes has not been investigated in detail. To identify the determinants of protein targeting to the inner envelope membrane (IM), we investigated the targeting of the nucleus-encoded integral IM protein, atTic40. We found that pre-atTic40 is imported into chloroplasts and processed to an intermediate size (int-atTic40) before insertion into the IM. Int-atTic40 is soluble and inserts into the IM from the internal stromal compartment. We also show that atTic40 and a second IM protein, atTic110, can target and insert into isolated IM vesicles in vitro. Collectively, our experiments are consistent with a “postimport” mechanism in which the IM proteins are first imported from the cytoplasm and subsequently inserted into the IM from the stroma.Keywords
This publication has 52 references indexed in Scilit:
- A Second Thylakoid Membrane-localized Alb3/OxaI/YidC Homologue Is Involved in Proper Chloroplast Biogenesis in Arabidopsis thalianaPublished by Elsevier BV ,2006
- A chloroplastic inner envelope membrane protease is essential for plant developmentFEBS Letters, 2006
- Evolution of Mitochondrial Oxa Proteins from Bacterial YidCJournal of Biological Chemistry, 2005
- ChloroP, a neural network‐based method for predicting chloroplast transit peptides and their cleavage sitesProtein Science, 1999
- Sorting of Nuclear-encoded Chloroplast Membrane Proteins to the Envelope and the Thylakoid MembranePublished by Elsevier BV ,1995
- The N-terminal hydrophobic region of the mature phosphate translocator is sufficient for targeting to the chloroplast inner envelope membrane.Plant Cell, 1995
- Identification of intermediates in the pathway of protein import into chloroplasts and their localization to envelope contact sites.The Journal of cell biology, 1993
- Cryopreservation of Chloroplasts and Thylakoids for Studies of Protein Import and IntegrationPlant Physiology, 1991
- Freeze-fracture electron microscopic analysis of ultrarapidly frozen envelope membranes on intact chloroplasts and after purificationProtoplasma, 1985
- Thermolysin Is a Suitable Protease for Probing the Surface of Intact Pea ChloroplastsPlant Physiology, 1984