Analysis of C‐glycosyl Flavonoids from South American Passiflora Species by HPLC‐DAD and HPLC‐MS
- 21 August 2011
- journal article
- research article
- Published by Wiley in Phytochemical Analysis
- Vol. 23 (3), 232-239
- https://doi.org/10.1002/pca.1348
Abstract
Introduction Leaves and fruits of Passiflora species are widely used around the world in popular medicine, mainly as sedatives and tranquilisers. C-glycosyl flavonoids are the main components of these species. Objective To investigate the constituent patterns and to develop a chromatographic method for the characterisation of the C-glycosyl flavonoids profile of the extracts of the leaves and the pericarp of South American Passiflora species. Methodology The chemical composition of extracts from the leaves and the fruits' pericarp of Passiflora edulis var. flavicarpa, P. edulis var. edulis, Passiflora alata, Passiflora tripartita var. mollissima, Passiflora quadrangularis, Passiflora manicata and Passiflora ligularis was evaluated for the presence of C-glycosyl flavonoids. Two separate HPLC methods were developed suitable for a diode array detector (DAD) and a MS detector. Separation by HPLC-DAD was achieved on a Luna C-18 column, using solvent A (tetrahydrofuran–isopropanol–acetonitrile) and solvent B (H3PO4 0.5%) in an isocratic elution mode. In the HPLC-MS, the components were separated on a Luna RP-18A column by a gradient elution (water–acetonitrile–formic acid). Results The presence of C-glycosyl flavonoids was identified in leaves and pericarp of P. edulis var. flavicarpa, P. alata, P. edulis var. edulis and P. tripartita var. molissima, but only in leaf extracts of P. quadrangularis and P. manicata and not at all in P. ligularis. The different species and varieties showed different major constituents. The C-glycosyl flavonoids identified more frequently were orientin, isoorientin, vitexin and isovitexin. Conclusion The methods established are simple and can be used as a tool for the characterisation and quality control of pharmaceutical preparations containing these Passiflora extracts. Copyright © 2011 John Wiley & Sons, Ltd.Funding Information
- CNPq
- CAPES
- DIB-Universidad Nacional de Colombia
This publication has 20 references indexed in Scilit:
- Neuropharmacological evaluation of the putative anxiolytic effects ofPassiflora edulis Sims, its sub-fractions and flavonoid constituentsPhytotherapy Research, 2006
- Antihypertensive Effect of an Extract ofPassiflora edulisRind in Spontaneously Hypertensive RatsBioscience, Biotechnology, and Biochemistry, 2006
- LC and UV determination of flavonoids from Passiflora alata medicinal extracts and leavesJournal of Pharmaceutical and Biomedical Analysis, 2005
- Chemical investigation and effects of the tea of Passiflora alata on biochemical parameters in ratsJournal of Ethnopharmacology, 2005
- Passiflora: a review updateJournal of Ethnopharmacology, 2004
- High-Speed Extraction and HPLC Fingerprinting of Medicinal Plants – I. Application to Passiflora FlavonoidsPharmaceutical Biology, 2002
- Comparative biological activity study on Passiflora incarnata and P. edulisFitoterapia, 2001
- The 6-C-chinovoside and 6-C-fucoside of luteolin from Passiflora edulisPhytochemistry, 1991
- Assessment of the hypnotic/sedative effects and toxicity ofPassiflora edulis aqueous extract in rodents and humansPhytotherapy Research, 1991
- PHARMAKOCHEMISCHE UNTERSUCHUNGEN DER DROGEN DER GATTUNG PASSIFLORAPlanta Medica, 1975