A high level of antibiotic resistance in Klebsiella and Aeromonas isolates from street water sold in Mozambique, associated with the prevalence of extended-spectrum and AmpC ß-lactamases
- 23 May 2022
- journal article
- research article
- Published by Taylor & Francis Ltd in Journal of Environmental Science and Health, Part B
- Vol. 57 (7), 561-567
- https://doi.org/10.1080/03601234.2022.2078627
Abstract
This study aims to evaluate the resistance profile and the prevalence of antibiotic resistance genes in 30 isolates of Klebsiella spp. and Aeromonas spp. recovered from water sold in the streets of Maputo. Susceptibility profiles to 15 antibiotics were performed according to Clinical Laboratory Standard Institute guidelines with antibiotic disks on Mueller-Hinton agar plates. Multiplex PCRs were performed targeting 10 ß-lactamase genes, five ESBL (blaTEM-variants, blaOXA-variants, BlaSHV-variants, MCTX-M Group 1 and Group 9 variants) and five AmpC (ACC variants, FOX variants, MOX variants, CIT variants and DHA variants). The results showed a high prevalence of Klebsiella resistance to ß-lactam antibiotics, such as amoxicillin/clavulanic acid (62.5%), amoxicillin (56.3%), ampicillin (50%), cefoxitin (43.8%), and cefotaxime (43.8%). Aeromonas showed resistance to cefoxitin and ampicillin (71.4%), amoxicillin/clavulanic acid (57.1%) and imipenem (42.9%). ESBL blaOXA-variants, blaSVH-variants, MCTX-M Group 1 variants, and MCTX-M Group 9 variants were the most prevalent b-lactam genes, followed by the b-lactams AmpC, ACC variants and FOX variants. It is extremely important to improve waterborne disease control strategies, especially in terms of public awareness of the potential health implications of multidrug-resistant strains of Klebsiella and Aeromonas, which are often neglected.Keywords
Funding Information
- FCT – Foundation for Science and Technology, I.P. (PRT/BD/151521/2021, UIDB/04129/2020)
- LEAF-Linking Landscape, Environment, Agriculture and Food, Research Unit
This publication has 40 references indexed in Scilit:
- Aeromonas spp. simultaneously harbouring blaCTX-M-15, blaSHV-12, blaPER-1 and blaFOX-2, in wild-growing Mediterranean mussel (Mytilus galloprovincialis) from Adriatic Sea, CroatiaInternational Journal of Food Microbiology, 2013
- Antimicrobial resistance profile ofAeromonasspecies isolated from LibyaLibyan Journal of Medicine, 2013
- Complexity of β-lactamases among clinical Aeromonas isolates and its clinical implicationsJournal of Microbiology, Immunology and Infection, 2012
- Unleashing Entrepreneurs or Controlling Unruly Providers? The Formalisation of Small-scale Water Providers in Greater Maputo, MozambiqueThe Journal of Development Studies, 2012
- A framework for global surveillance of antibiotic resistanceDrug Resistance Updates, 2011
- Unreliable Extended-Spectrum β-Lactamase Detection in the Presence of Plasmid-Mediated AmpC in Escherichia coli Clinical IsolatesJournal of Clinical Microbiology, 2009
- Detection of plasmid-mediated class C β-lactamasesInternational Journal of Infectious Diseases, 2007
- Microbial Diversity in Water and Sediment of Lake Chaka, an Athalassohaline Lake in Northwestern ChinaApplied and Environmental Microbiology, 2006
- Prevalence and risk factors of extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae in an Israeli long-term care facilityEuropean Journal of Clinical Microbiology & Infectious Diseases, 2004
- Isolation of a Chromosomal Region ofKlebsiella pneumoniaeAssociated with Allantoin Metabolism and Liver InfectionInfection and Immunity, 2004