Development of a Drug Delivery System Based on Chitosan Nanoparticles for Oral Administration of Interferon-α
- 8 September 2017
- journal article
- research article
- Published by American Chemical Society (ACS) in Biomacromolecules
- Vol. 18 (10), 3302-3309
- https://doi.org/10.1021/acs.biomac.7b00959
Abstract
Despite the good clinical efficacy of interferon-alpha (IFNα) to treat some types of cancer and viral infections, this biological drug is underused given its severe adverse effects and high dosing parenteral regimens. Aiming to achieve a breakthrough in therapy with IFNα, this work reports for the first time on the design and full characterization of a novel nanomedicine of IFNα-2b-loaded chitosan nanoparticles (IFN-CT NPs) for oral delivery. IFN-CT NPs produced by ionotropic gelation, encapsulating approximately 100% of the drug, showed a size of 36 ± 8 nm, zeta potential of +30 mV (dynamic light scattering), and spherical morphology (transmission electron microscopy). The antiviral activity of IFN-CT NPs in vitro was comparable to that of commercial IFNα. Remarkably, both treatments stimulated the expression of IFN response genes to a similar extent in both noninfected and infected cells with Human Lymphotropic-T Virus type 1. Finally, oral administration of IFN-CT NPs (0.3 MIU) to CF1 mice showed detectable levels of IFNα in plasma after 1 h, whereas no IFNα was detected with a commercial formulation. These results are encouraging and open a new avenue for the administration of this biological drug in a minimally invasive, safer, and more patient-compliant way.Keywords
Funding Information
- Consejo Nacional de Investigaciones Cient?ficas y T?cnicas (PIP 112 20110100644)
- Fondo para la Investigaci?n Cient?fica y Tecnol?gica (PICT 2012-0322)
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