Separation of [1-3H]cellooligosaccharides by thin-layer chromatography: Assay for cellulolytic enzymes
- 1 November 1985
- journal article
- research article
- Published by Elsevier BV in Analytical Biochemistry
- Vol. 150 (2), 264-272
- https://doi.org/10.1016/0003-2697(85)90509-3
Abstract
A thin-layer chromatographic method for the separation with high resolution of [1-3H]-cellooligosaccharides on silica gel plates has been developed. Reducing end-labeled glucose through cellohexaose were separated on silica gel plates with three ascents of ethyl acetate:water methanol (40:15:20;v:v) and each was extracted with an efficiency of 88 .+-. 3%. Separations of cellooligosaccharides using other adsorbents, solvents, and impregnants are also described. This thin-layer chromatographic method facilitated analysis of the activity of cellulolytic enzymes.Keywords
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