Human immunodeficiency virus has an aspartic-type protease that can be inhibited by pepstatin A.
- 1 September 1988
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 85 (18), 6612-6616
- https://doi.org/10.1073/pnas.85.18.6612
Abstract
The protease encoded by the human immunodeficiency virus (HIV) processes the viral gag and gag-pol protein precursor by posttranslational cleavage. In this study we have demonstrated by site-specific mutagenesis (Asp .fwdarw. Thr) and by pepstatin A inhibition that the recombinant HIV protease is an aspartic-type- protease. Furthermore, incubation of HIV-infected H9 cells with pepstatin A inhibited part of the intracellular processing of the HIV gag protein yet had no apparent toxicity on HIV-infected cells during 48 hr of incubation.This publication has 40 references indexed in Scilit:
- Inhibition of retroviral protease activity by an aspartyl proteinase inhibitorNature, 1987
- The Efficacy of Azidothymidine (AZT) in the Treatment of Patients with AIDS and AIDS-Related ComplexNew England Journal of Medicine, 1987
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Molecular Characterization of Human T-Cell Leukemia (Lymphotropic) Virus Type III in the Acquired Immune Deficiency SyndromeScience, 1984
- Detection, Isolation, and Continuous Production of Cytopathic Retroviruses (HTLV-III) from Patients with AIDS and Pre-AIDSScience, 1984
- Potent new inhibitors of human reninNature, 1982
- Effect of Protease Inhibitors on the Activation of Epstein-Barr Virus Repressed in Cultured Lymphoid CellsIntervirology, 1980
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Double-blind randomised clinical trial of a pepsin-inhibitory pentapeptide (pepstatin) in the treatment of duodenal ulcer.Gut, 1979
- Properties of a P70 proteolytic factor of murine leukemia virusesCell, 1977