Histone-Dependent Association of Tup1-Ssn6 with Repressed Genes In Vivo
- 1 February 2002
- journal article
- research article
- Published by Informa UK Limited in Molecular and Cellular Biology
- Vol. 22 (3), 693-703
- https://doi.org/10.1128/mcb.22.3.693-703.2002
Abstract
The Tup1-Ssn6 complex regulates diverse classes of genes in Saccharomyces cerevisiae and serves as a model for corepressor functions in many organisms. Tup1-Ssn6 does not directly bind DNA but is brought to target genes through interactions with sequence-specific DNA binding factors. Full repression by Tup1-Ssn6 appears to require interactions with both the histone tails and components of the general transcription machinery, although the relative contribution of these two pathways is not clear. Here, we map Tup1 locations on two classes of Tup1-Ssn6-regulated genes in vivo via chromatin immunoprecipitations. Distinct profiles of Tup1 are observed on a cell-specific genes and DNA damage-inducible genes, suggesting that alternate repressive architectures may be created on different classes of repressed genes. In both cases, decreases in acetylation of histone H3 colocalize with Tup1. Strikingly, although loss of the Srb10 mediator protein had no effect on Tup1 localization, both histone tail mutations and histone deacetylase mutations crippled the association of Tup1 with target loci. Together with previous findings that Tup1-Ssn6 physically associates with histone deacetylase activities, these results indicate that the repressor complex alters histone modification states to facilitate interactions with histones and that these interactions are required to maintain a stable repressive state.Keywords
This publication has 49 references indexed in Scilit:
- SMRTe Inhibits MEF2C Transcriptional Activation by Targeting HDAC4 and 5 to Nuclear DomainsJournal of Biological Chemistry, 2001
- Role of Histone H3 Lysine 9 Methylation in Epigenetic Control of Heterochromatin AssemblyScience, 2001
- Hrs1/Med3 Is a Cyc8-Tup1 Corepressor Target in the RNA Polymerase II HoloenzymePublished by Elsevier BV ,2000
- The language of covalent histone modificationsNature, 2000
- A Complex Composed of Tup1 and Ssn6 Represses Transcription in VitroPublished by Elsevier BV ,1997
- Repression domain of the yeast global repressor Tup1 interacts directly with histones H3 and H4.Genes & Development, 1996
- The tetratricopeptide repeats of Ssn6 interact with the homeo domain of alpha 2.Genes & Development, 1995
- Distinct TPR motifs of Cyc8 are involved in recruiting the Cyc8-Tup1 corepressor complex to differentially regulated promoters.Genes & Development, 1995
- The WD repeats of Tup1 interact with the homeo domain protein alpha 2.Genes & Development, 1994
- Functional dissection of the yeast Cyc8–Tupl transcriptional co-repressor complexNature, 1994