High clustering rates of multidrug-resistant Mycobacterium tuberculosisgenotypes in Panama
Open Access
- 23 September 2013
- journal article
- Published by Springer Science and Business Media LLC in BMC Infectious Diseases
- Vol. 13 (1), 442
- https://doi.org/10.1186/1471-2334-13-442
Abstract
Tuberculosis continues to be one of the leading causes of death worldwide and in the American region. Although multidrug-resistant tuberculosis (MDR-TB) remains a threat to TB control in Panama, few studies have focused in typing MDR-TB strains. The aim of our study was to characterize MDR Mycobacterium tuberculosis clinical isolates using PCR-based genetic markers. From 2002 to 2004, a total of 231 Mycobacterium tuberculosis isolates from TB cases country-wide were screened for antibiotic resistance, and MDR-TB isolates were further genotyped by double repetitive element PCR (DRE-PCR), (GTG)5-PCR and spoligotyping. A total of 37 isolates (0.85%) were resistant to both isoniazid (INH) and rifampicin (RIF). Among these 37 isolates, only two (5.4%) were resistant to all five drugs tested. Dual genotyping using DRE-PCR and (GTG)5-PCR of MDR Mycobacterium tuberculosis isolates revealed eight clusters comprising 82.9% of the MDR-TB strain collection, and six isolates (17.1%) showed unique fingerprints. The spoligotyping of MDR-TB clinical isolates identified 68% as members of the 42 (LAM9) family genotype. Our findings suggest that MDR Mycobacterium tuberculosis is highly clustered in Panama’s metropolitan area corresponding to Panama City and Colon City, and our study reveals the genotype distribution across the country.Keywords
This publication has 20 references indexed in Scilit:
- Inferring patient to patient transmission of Mycobacterium tuberculosisfrom whole genome sequencing dataBMC Infectious Diseases, 2013
- Use of Multiplex Allele-Specific Polymerase Chain Reaction (MAS-PCR) to Detect Multidrug-Resistant Tuberculosis in PanamaPLOS ONE, 2012
- Tuberculosis in Sudan: a study of Mycobacterium tuberculosis strain genotype and susceptibility to anti-tuberculosis drugsBMC Infectious Diseases, 2011
- Multidrug-Resistant Mycobacterium tuberculosis, Southwestern ColombiaEmerging Infectious Diseases, 2011
- Mycobacterium tuberculosis spoligotypes and drug susceptibility pattern of isolates from tuberculosis patients in South-Western UgandaBMC Infectious Diseases, 2011
- Extensive transmission of isoniazid resistant M. tuberculosis and its association with increased multidrug-resistant TB in two rural counties of eastern China: A molecular epidemiological studyBMC Infectious Diseases, 2010
- Genotyping and drug resistance patterns of M. tuberculosis strains in PakistanBMC Infectious Diseases, 2008
- Multi drug resistant tuberculosis: a challenge in the management of tuberculosisAfrican Journal of Health Sciences, 2008
- Molecular and Conventional Epidemiology of Tuberculosis in Hong Kong: a Population-Based Prospective StudyJournal of Clinical Microbiology, 2003
- Molecular epidemiology of tuberculosisEuropean Respiratory Journal, 2002