General Method for Modification of Liposomes for Encoded Assembly on Supported Bilayers

Abstract

An amphiphilic oligonucleotide species ((C₁₈)₂-DNA) is presented as a generally useful reagent to display encoded tether sequences on the surface of phospholipid assemblies. (C₁₈)₂-DNA inserts into preformed vesicles and proteoliposomes of arbitrary composition, content, and origin using a simple and gentle procedure and is a significant improvement over the previously described method particularly since it allows postmodification of any phospholipid assembly without the need for special lipids carrying reactive headgroups. DNA-modified vesicles can then be tethered, via DNA hybridization, onto a supported phospholipid bilayer displaying the complementary sequence. The encoding capability of the tether can be exploited to form an array of tethered vesicles spatially defined by the DNA sequence displayed on the surface and demonstrates that (C₁₈)₂-DNA is stably associated with a membrane to allow sorting. Vesicles tethered in this way show two-dimensional mobility, reflecting the fluidity of the supporting bilayer, and promises to be a useful system with which to study vesicle−vesicle interactions.