Evidence for Bioavailable Copper−Dissolved Organic Matter Complexes and Transiently Increased Copper Bioavailability in Manure-Amended Soils as Determined by Bioluminescent Bacterial Biosensors
- 18 March 2008
- journal article
- Published by American Chemical Society (ACS) in Environmental Science & Technology
- Vol. 42 (8), 3102-3108
- https://doi.org/10.1021/es071916+
Abstract
The short-term (3 months) dynamics of bioavailable copper (Cu) species was determined in soils amended with various amounts of manure and Cu. Bioavailable Cu species were operationally defined as those species that were able to induce gene expression in a Cu-specific Pseudomonas fluorescens biosensor. Biosensor measurements were backed by analysis of total Cu in soil and of total Cu and free Cu2+ ion activity in solution. Cu bioavailability relative to the total Cu concentration increased dramatically with increasing Cu loading of manure and with increasing manure amendment to soil. In both cases, the immediate increase in bioavailability could be explained in part by increased Cu concentration in solution and in part by an increased bioavailability of dissolved Cu species. In contrast to Cu bioavailability, Cu2+ ion activity decreased progressively with increasing manure loading. Cu bioavailability declined rapidly during the weeks after manure amendment concomitant with a marked slow-down of C mineralization indicating a shift from initially bioavailable Cu-dissolved organic matter (Cu-DOM) complexes to nonavailable Cu-DOM complexes over time. Our data do not support the conventional view of metal bioavailability being primarily related to the free metal ion activity and strongly suggest differential bioavailability of Cu-DOM complexes in manure-amended soils.Keywords
This publication has 36 references indexed in Scilit:
- Use of an ion-selective electrode for free copper measurements in low salinity and low ionic strength matricesEnvironmental Chemistry, 2007
- Decreased abundance and diversity of culturable Pseudomonas spp. populations with increasing copper exposure in the sugar beet rhizosphereFEMS Microbiology Ecology, 2006
- Detection of Copper(II) and zinc(II) binding to humic acids from pig slurry and amended soils by fluorescence spectroscopyEnvironmental Pollution, 2006
- Illuminating the detection chain of bacterial bioreportersEnvironmental Microbiology, 2004
- Degradation of low molecular weight organic acids complexed with heavy metals in soilGeoderma, 2004
- Effects of copper amendment on the bacterial community in agricultural soil analyzed by the T-RFLP techniqueFEMS Microbiology Ecology, 2003
- Distribution and bioavailability of copper in farm effluentScience of The Total Environment, 2003
- Activity and population dynamics of heterotrophic and ammonia‐oxidizing microorganisms in soil surrounding sludge bands spiked with linear alkylbenzene sulfonate: A field studyEnvironmental Toxicology and Chemistry, 2003
- Derivation of soil quality criteria using predicted chemical speciation of Pb2+ and Cu2+Environmental Toxicology and Chemistry, 1998
- Copper Activity in Soil Solution: I. Measurement by Ion‐selective Electrode and Donnan DialysisSoil Science Society of America Journal, 1987