Detection of DNA Strand Breaks in Single Cells Using Flow Cytometry
- 1 January 1984
- journal article
- research article
- Published by Taylor & Francis Ltd in International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine
- Vol. 46 (5), 521-527
- https://doi.org/10.1080/09553008414551731
Abstract
A preliminary method is reported of alkaline unwinding of DNA within single cells and quantitation of the single-stranded and double-stranded DNA with the fluorescent probe acridine orange. A suspension of alkali-treated cells is obtained and analysed by flow cytometry. An increase in the amount of single-stranded DNA is taken as an indication of strand breaks. An advantage of this method is that a large number of cells can be individually analysed for DNA strand breaks. A measurement of DNA content is also obtained, making it possible to discriminate between cells in various parts of the cell cycle.Keywords
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