Estimation of biological chromophores using diffuse optical spectroscopy: benefit of extending the UV-VIS wavelength range to include 1000 to 1600 nm
Open Access
- 17 November 2010
- journal article
- research article
- Published by Optica Publishing Group in Biomedical Optics Express
- Vol. 1 (5), 1432-1442
- https://doi.org/10.1364/boe.1.001432
Abstract
With an optical fiber probe, we acquired spectra from swine tissue between 500 and 1600 nm by combining a silicon and an InGaAs spectrometer. The concentrations of the biological chromophores were estimated by fitting a mathematical model derived from diffusion theory. The advantage of our technique relative to those presented in previous studies is that we extended the commonly-used wavelength ranges of 500 and 1000 nm to include the range of 1000 to 1600 nm, where additional water and lipid absorption features exist. Hence, a more accurate estimation of these two chromophores is expected when spectra are fitted between 500 and 1600 nm than between 500 and 1000 nm. When extending the UV-VIS wavelength range, the estimated total amount of chromophores approached 100% of the total as present in the probed volume. The confidence levels of the water and lipid related parameters increases by a factor of four.Keywords
This publication has 29 references indexed in Scilit:
- Quantitative Optical Spectroscopy: A Robust Tool for Direct Measurement of Breast Cancer Vascular Oxygenation and Total Hemoglobin Content In vivoCancer Research, 2009
- Diagnosing breast cancer using diffuse reflectance spectroscopy and intrinsic fluorescence spectroscopyJournal of Biomedical Optics, 2008
- In vivo absorption, scattering, and physiologic properties of 58 malignant breast tumors determined by broadband diffuse optical spectroscopyJournal of Biomedical Optics, 2006
- Monitoring PDT by means of superficial reflectance spectroscopyJournal of Photochemistry and Photobiology B: Biology, 2005
- Measurement of Hypoxia-related Parameters in Bronchial Mucosa by Use of Optical SpectroscopyAmerican Journal of Respiratory and Critical Care Medicine, 2005
- In vivo measurement of the local optical properties of tissue by use of differential path-length spectroscopyOptics Letters, 2004
- Optical biopsy of bone tissue: a step toward the diagnosis of bone pathologiesJournal of Biomedical Optics, 2004
- Frequency-domain multichannel optical detector for noninvasive tissue spectroscopy and oximetryOptical Engineering, 1995
- Multispectral tissue characterization with time-resolved detection of diffusely scattered white lightOptics Letters, 1993
- A diffusion theory model of spatially resolved, steady-state diffuse reflectance for the noninvasive determination of tissue optical properties in vivoMedical Physics, 1992