Regulation of a Cyclin-CDK-CDK Inhibitor Complex by Inositol Pyrophosphates
- 6 April 2007
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 316 (5821), 109-112
- https://doi.org/10.1126/science.1139080
Abstract
In budding yeast, phosphate starvation triggers inhibition of the Pho80-Pho85 cyclin–cyclin-dependent kinase (CDK) complex by the CDK inhibitor Pho81, leading to expression of genes involved in nutrient homeostasis. We isolated myo - d -inositol heptakisphosphate (IP 7 ) as a cellular component that stimulates Pho81-dependent inhibition of Pho80-Pho85. IP 7 is necessary for Pho81-dependent inhibition of Pho80-Pho85 in vitro. Moreover, intracellular concentrations of IP 7 increased upon phosphate starvation, and yeast mutants defective in IP 7 production failed to inhibit Pho80-Pho85 in response to phosphate starvation. These observations reveal regulation of a cyclin-CDK complex by a metabolite and suggest that a complex metabolic network mediates signaling of phosphate availability.This publication has 26 references indexed in Scilit:
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