Cytotoxic Activity of Six Samples of Brazilian Propolis on Sea Urchin (Lytechinus variegatus) Eggs
Open Access
- 1 January 2013
- journal article
- research article
- Published by Hindawi Limited in Evidence-Based Complementary and Alternative Medicine
- Vol. 2013, 1-4
- https://doi.org/10.1155/2013/619361
Abstract
The cytotoxic activities of extracts of four samples of propolis from the state of Minas Gerais (Southeast Brazil) and two from the state of Paraná (South Brazil) were evaluated using sea urchin (Lytechinus variegatus) eggs. Cytotoxic activity was observed, characterized mainly by the inhibition of the first cleavage of newly fertilized eggs. Methanol extracts at 32 µg mL−1of all samples were highly active (97–100%). Extracts were also prepared by successive treatments of the samples with hexane, chloroform, ethyl acetate, and methanol. High activity was observed using the ethyl acetate fractions of all samples, but hexane and chloroform fractions of some samples also had high activity. Based on the chemical composition of the extracts and fractions (published previously), it is hypothesized that the cytotoxic activities observed are due mainly to artepillin C,p-coumaric acid, and kaempferide. The results suggest that caffeoylquinic acids have no cytotoxic activity in sea urchin eggs.Funding Information
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
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