Effect of glycine on the calcium signal of thrombin-stimulated platelets
- 1 June 2007
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in Blood Coagulation & Fibrinolysis
- Vol. 18 (4), 303-308
- https://doi.org/10.1097/mbc.0b013e3281223535
Abstract
In treatment of hemorrhagic shock, small-volume infusion of 7.5% NaCl gives immediate hemodynamic improvement, but in vitro experiments suggest it depresses the hemostatic system. Since previous reports showed that hyperosmotic glycine solutions preserved the platelet function better than hyperosmotic NaCl solutions, we investigated whether glycine changes the intracellular calcium ([Ca2+]i) signal. Platelets were incubated in hyperosmotic solutions containing sodium glycine or glycine base and stimulated with 0.1 IU/ml thrombin. [Ca2+]i increases were compared with an isosmotic control. Platelets incubated in zero calcium/EGTA were used to study separately the effect of glycine on calcium mobilization from intracellular stores and extracellular calcium entry. When NaCl was replaced by sodium glycine, the [Ca2+]i increase produced by thrombin was enhanced, because the calcium entry increased without changes in the mobilization of stored calcium. The addition of 50 mmol/l glycine base to the HEPES-buffered media increases the thrombin-induced entry of calcium or manganese. This study demonstrates that hyperosmotic glycine solutions increase the entry of calcium. This effect contrasts with the impairment of the thrombin-induced calcium signals by NaCl. The addition of low amounts of glycine in resuscitation solutions would be useful to reduce dysfunctional inflammatory responses without the risk of bleeding; however, concentrated solutions could cause toxic effects.Keywords
This publication has 19 references indexed in Scilit:
- A comparison of the effect of 1.5% glycine and 5% glucose irrigants on plasma serum physiology and the incidence of transurethral resection syndrome during prostate resectionBJU International, 2005
- Hypertonic saline solutions attenuate agonist-induced changes of intracellular calciumPlatelets, 2004
- Effect of hyperosmolarity on agonist-induced increases of intracellular calcium in human plateletsThrombosis Research, 2003
- Hypertonic resuscitation and blood coagulation: In vitro comparison of several hypertonic solutions for their action on platelets and plasma coagulationThrombosis Research, 2002
- Clinical review: Hypertonic saline resuscitation in sepsisCritical Care, 2002
- Glycine‐gated chloride channels in neutrophils attenuate calcium influx and superoxide productionThe FASEB Journal, 2000
- GLYCINE IMPROVES SURVIVAL AFTER HEMORRHAGIC SHOCK IN THE RATShock, 1999
- Influence of glycine on the damage induced in isolated perfused rat liver by five hepatotoxic agentsToxicology, 1998
- A diet containing glycine improves survival in endotoxin shock in the ratAmerican Journal of Physiology-Gastrointestinal and Liver Physiology, 1996
- Hypertonic Saline Alters Plasma Clotting Times and Platelet AggregationJournal Of Trauma-Injury Infection and Critical Care, 1991