Neutrophils influence melanoma adhesion and migration under flow conditions
Open Access
- 18 June 2003
- journal article
- research article
- Published by Wiley in International Journal of Cancer
- Vol. 106 (5), 713-722
- https://doi.org/10.1002/ijc.11297
Abstract
We have studied human melanoma cell (C8161) adhesion and migration in response to stimulation by soluble collagen IV (CIV) using a modified Boyden chamber. In this modified chamber, shear flow can be introduced over the cell‐substrate interface, affecting tumor cell chemotactic migration through a microporous filter. A relatively high level of intercellular adhesion molecule‐1 (ICAM‐1) was found on C8161 cells. In contrast, levels of β2‐integrins (e.g., LFA‐1 and Mac‐1), the molecules that would be necessary for C8161 stable adhesion to the endothelium substrate, were found to be very low on these melanoma cells. As a result, C8161 transendothelial migration under a flow condition of 4 dyn/cm2 decreased by 70% as compared to static migration. When human neutrophils (PMNs) were present in the tumor cell suspension, C8161 migration recovered by 85% over C8161 cells alone under the 4 dyn/cm2 flow condition. Blocking ICAM‐1 on C8161 cells or Mac‐1 on PMNs significantly inhibited C8161‐PMN adhesion and subsequent C8161 migration through the endothelium under flow conditions. In addition, increased interleukin‐8 production and Mac‐1 expression by PMNs were detected when they were co‐cultured with C8161 melanoma cells. These results suggest that transmigration of C8161 cells under flow conditions can be influenced by PMNs, mediated by Mac‐1/ICAM‐1 adhesive interactions and enhanced by altered cytokine production.Keywords
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