Cloning and Characterization of Two Lactobacillus casei Genes Encoding a Cystathionine Lyase
- 1 January 2008
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 74 (1), 99-106
- https://doi.org/10.1128/aem.00745-07
Abstract
Volatile sulfur compounds are key flavor compounds in several cheese types. To better understand the metabolism of sulfur-containing amino acids, which certainly plays a key role in the release of volatile sulfur compounds, we searched the genome database of Lactobacillus casei ATCC 334 for genes encoding putative homologs of enzymes known to degrade cysteine, cystathionine, and methionine. The search revealed that L. casei possesses two genes that putatively encode a cystathionine β-lyase (CBL; EC 4.4.1.8). The enzyme has been implicated in the degradation of not only cystathionine but also cysteine and methionine. Recombinant CBL proteins catalyzed the degradation of l -cystathionine, O -succinyl- l -homoserine, l -cysteine, l -serine, and l -methionine to form α-keto acid, hydrogen sulfide, or methanethiol. The two enzymes showed notable differences in substrate specificity and pH optimum.Keywords
This publication has 34 references indexed in Scilit:
- Role of Cystathionine β-Lyase in Catabolism of Amino Acids to Sulfur Volatiles by Genetic Variants of Lactobacillus helveticus CNRZ 32Applied and Environmental Microbiology, 2007
- Characterisation of the non-starter lactic acid bacteria (NSLAB) of Gruyère PDO cheeseLe Lait, 2006
- Comparative genomics of the lactic acid bacteriaProceedings of the National Academy of Sciences of the United States of America, 2006
- YtjE from Lactococcus lactis IL1403 Is a C-S Lyase with α,γ-Elimination Activity toward MethionineApplied and Environmental Microbiology, 2006
- Flavour formation by amino acid catabolismBiotechnology Advances, 2006
- Sulfur Amino Acid Metabolism and Its Control in Lactococcus lactis IL1403Journal of Bacteriology, 2005
- Identification and characterization of a cystathionine β/γ-lyase from Lactococcus lactis ssp. cremoris MG1363FEMS Microbiology Letters, 2000
- Purification and characterization of cystathionine γ-lyase from Lactobacillus fermentum DT41FEMS Microbiology Letters, 1998
- Purification and characterization of cystathionine γ-lyase fromLactobacillus fermentumDT41FEMS Microbiology Letters, 1998
- A MEDIUM FOR THE CULTIVATION OF LACTOBACILLIJournal of Applied Bacteriology, 1960