Micellar electrokinetic chromatography for high‐performance analytical separation
- 1 January 2008
- journal article
- review article
- Published by Wiley in The Chemical Record
- Vol. 8 (5), 291-301
- https://doi.org/10.1002/tcr.20156
Abstract
Capillary electrophoresis (CE) is a relatively new method of analytical separation having the advantages of high separation efficiency, requirement of a small sample amount, low operating cost, and fast separation time. CE is a separation method where the analyte migrates under an electric field due to a charge on the analyte. Hence, CE was unable to separate neutral analytes until the advent of micellar electrokinetic chromatography (MEKC). MEKC is performed with an addition of ionic micelles to an electrophoretic medium, where a portion of the analyte is incorporated into the micelle and has an apparent charge, which can be subject to electrophoretic separation. The migration velocity of the neutral analyte in MEKC depends on what portion of the analyte is incorporated into the micelle. Thus, the separation principle of MEKC is similar to that of chromatography, although the micelle corresponding to the stationary phase in chromatography is not stationary inside the capillary. The fundamental characteristics and theoretical treatments of the behavior of the analyte in MEKC were studied extensively by the author's group. MEKC has been established as one of the most popular separation modes in CE. This review describes how MEKC was developed and how it is useful as a method of analytical separation. © 2008 The Japan Chemical Journal Forum and Wiley Periodicals, Inc. Chem Rec 8: 291–301; 2008: Published online in Wiley InterScience ( www.interscience.wiley.com) DOI 10.1002/tcr.20156Keywords
This publication has 42 references indexed in Scilit:
- CaSSS Award: J. Sep. Sci. 1/2008Journal of Separation Science, 2008
- Application of sweeping to micellar electrokinetic chromatography-atmospheric pressure chemical ionization-mass spectrometric analysis of environmental pollutantsElectrophoresis, 2001
- Micellar Electrokinetic ChromatographyBulletin of the Chemical Society of Japan, 1998
- Micelle Polymers as Pseudostationary Phases in MEKC: Chromatographic Performance and Chemical SelectivityAnalytical Chemistry, 1997
- Novel pseudostationary phase for micellar electrokinetic capillary chromatographyJournal of Microcolumn Separations, 1992
- Optimization of micellar electrokinetic chromatographyAnalytical Chemistry, 1990
- Ion-exchange electrokinetic chromatography with polymer ions for the separation of isomeric ions having identical electrophoretic mobilitiesAnalytical Chemistry, 1990
- Optical resolution of enantiomers with chiral mixed micelles by electrokinetic chromatographyAnalytical Chemistry, 1989
- Electrokinetic chromatography: An interface between electrophoresis and chromatographyTrAC Trends in Analytical Chemistry, 1989
- Free zone electrophoresisChromatographic Reviews, 1967