Salmonella type III effectors PipB and PipB2 are targeted to detergent‐resistant microdomains on internal host cell membranes
- 1 August 2003
- journal article
- research article
- Published by Wiley in Molecular Microbiology
- Vol. 49 (3), 685-704
- https://doi.org/10.1046/j.1365-2958.2003.03598.x
Abstract
The intracellular pathogen, Salmonella enterica, translocates type III effectors across its vacuolar membrane into host cells. Herein we describe a new Salmonella effector, PipB2, which has sequence similarity to another type III effector, PipB. In phagocytic cells, PipB2 localizes to the Salmonella-containing vacuole (SCV) and tubular extensions from the SCV, Salmonella-induced filaments (Sifs). We used the specific targeting of PipB2 in macrophages to characterize Sifs in phagocytic cells for the first time. In epithelial cells, PipB2 has a unique localization pattern, localizing to SCVs and Sifs and additionally to vesicles at the periphery of infected cells. We further show that the N-terminal 225-amino-acid residues of PipB2 are sufficient for type III translocation and association with SCVs and Sifs, but not peripheral vesicles. Subcellular fractionation demonstrated that both PipB and PipB2 associate with host cell membranes and resist extraction by high salt, high pH and to a significant extent, non-ionic detergent. Furthermore, PipB and PipB2 are enriched in detergent-resistant microdomains (DRMs), also known as lipid rafts, present on membranes of SCVs and Sifs. The enrichment of Salmonella effectors in DRMs on these intracellular membranes probably permits specific interactions with host cell molecules that are concentrated in these signalling platforms.Keywords
This publication has 97 references indexed in Scilit:
- hetL Overexpression Stimulates Heterocyst Formation in Anabaena sp. Strain PCC 7120Journal of Bacteriology, 2002
- The Vesicle- and Target-SNARE Proteins That Mediate Glut4 Vesicle Fusion Are Localized in Detergent-insoluble Lipid Rafts Present on Distinct Intracellular MembranesPublished by Elsevier BV ,2002
- SseF and SseG are translocated effectors of the type III secretion system ofSalmonellapathogenicity island 2 that modulate aggregation of endosomal compartmentsCellular Microbiology, 2002
- Presence of detergent-resistant microdomains in lysosomal membranesBiochemical and Biophysical Research Communications, 2002
- Type III Secretion of Salmonella enterica Serovar Typhimurium Translocated Effectors and SseFGInfection and Immunity, 2002
- Role of Lipid Modifications in Targeting Proteins to Detergent-resistant Membrane RaftsPublished by Elsevier BV ,1999
- FUNCTIONS OF LIPID RAFTS IN BIOLOGICAL MEMBRANESAnnual Review of Cell and Developmental Biology, 1998
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994
- In AtT20 and HeLa cells brefeldin A induces the fusion of tubular endosomes and changes their distribution and some of their endocytic properties.The Journal of cell biology, 1992
- Specific inhibition of phagosome-lysosome fusion in murine macrophages mediated bySalmonella typhimuriuminfectionFEMS Microbiology Letters, 1990