A comparative trial of anti-glycoconjugate antibody assays: IgM antibodies to GM1
- 1 January 1994
- journal article
- research article
- Published by Springer Science and Business Media LLC in Zeitschrift für Neurologie
- Vol. 241 (8), 475-480
- https://doi.org/10.1007/bf00919708
Abstract
IgM class antibodies against the ganglioside GM1 have been found in a subgroup of patients with lower motor neuron syndromes and multifocal motor neuropathies (MMN). The pathogenic relevance of these antibodies is still unclear, but some MMN patients with IgM antibodies against GM1 seem to profit from immunosuppressive therapy. A reliable test for IgM antibodies against GMl may be useful for identifying these patients. We have assessed the comparability of the ELISA tests used for the determination of IgM against GM1 by sending coded serum samples to nine laboratories. In three samples high-titre IgM antibodies against GM1 were detected by all laboratories. This result was confirmed by dot blot immunodetection and thin-layer chromatography immuno-overlay. Seven samples were read as negative by nearly all laboratories. Major discrepancies between laboratories were noted in the analysis of one sample with results ranging from negative to “high titre”.Keywords
This publication has 26 references indexed in Scilit:
- Conduction abnormalities induced by sera of patients with multifocal motor neuropathy and anti‐GM1 antibodiesMuscle & Nerve, 1993
- Antiganglioside antibodies in motor-neuron diseases and peripheral neuropathies: study by ELISA technique and immunodetection on thin-layer chromatographyNeurochemistry International, 1992
- Invited review: Motor neuropathies, motor neuron disorders, and antiglycolipid antibodiesMuscle & Nerve, 1991
- Clinical correlations of Anti-Gm1 antibodies in amyotrophic lateral sclerosis and neuropathiesMuscle & Nerve, 1991
- Immunosuppressive treatment in multifocal motor neuropathyAnnals of Neurology, 1991
- IgM deposits at nodes of ranvier in a patient with amyotrophic lateral sclerosis, anti‐GM1 antibodies, and multifocal motor conduction blockAnnals of Neurology, 1990
- Polyclonal IgM anti‐GM1 ganglioside antibody in patients with motor neuron disease and variantsAnnals of Neurology, 1990
- Lower motor neuron syndromes defined by patterns of weakness, nerve conduction abnormalities, and high titers of antiglycolipid antibodiesAnnals of Neurology, 1990
- Measurement and significance of antibodies against GM1 ganglioside Report of a workshop, 18 April 1989, Chicago, IL, U.S.A.Journal of Neuroimmunology, 1989
- A treatable multifocal motor neuropathy with antibodies to GM1 gangliosideAnnals of Neurology, 1988