Synthesis and Fermentation Properties of Novel Galacto-Oligosaccharides by β-Galactosidases from Bifidobacterium Species
Open Access
- 1 June 2001
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 67 (6), 2526-2530
- https://doi.org/10.1128/aem.67.6.2526-2530.2001
Abstract
β-Galactosidase enzymes were extracted from pure cultures of Bifidobacterium angulatum, B. bifidum BB-12, B. adolescentis ANB-7, B. infantis DSM-20088, and B. pseudolongum DSM-20099 and used in glycosyl transfer reactions to synthesize oligosaccharides from lactose. At a lactose concentration of 30% (wt/wt) oligosaccharide yields of 24.7 to 47.6% occurred within 7 h. Examination of the products by thin-layer chromatography and methylation analysis revealed distinct product derived spectra from each enzyme. These were found to be different to that of Oligomate 55, a commercial prebiotic galacto-oligosaccharide. Fermentation testing of the oligosaccharides showed an increase in growth rate, compared to Oligomate 55, with products derived from B. angulatum, B. bifidum, B. infantis , and B. pseudolongum . However B. adolescentis had a lower growth rates on its oligosaccharide compared with Oligomate 55. Mixed culture testing of the B. bifidum BS-4 oligosaccharide showed that the overall prebiotic effect was equivalent to that of Oligomate 55.Keywords
This publication has 20 references indexed in Scilit:
- Recent progress on research and applications of non-digestible galacto-oligosaccharidesInternational Dairy Journal, 1999
- Probiotics and prebiotics: microflora management for improved gut healthClinical Microbiology & Infection, 1998
- Functional food science and gastrointestinal physiology and functionBritish Journal of Nutrition, 1998
- Growth of a human intestinal Desulfovibrio desulfuricans in continuous cultures containing defined populations of saccharolytic and amino acid fermenting bacteriaJournal of Applied Microbiology, 1998
- Production, properties and applications of food-grade oligosaccharidesTrends in Food Science & Technology, 1996
- Characterization of a variant of the polysaccharide acetan produced by a mutant of Acetobacter xylinum strain CR1/4Journal of Applied Bacteriology, 1993
- An improved method for the preparation of standards for glycosyl-linkage analysis of complex carbohydratesCarbohydrate Research, 1991
- Effects of Administration of Galactooligosaccharides on the Human Faecal Microflora, Stool Weight and Abdominal SensationMicrobial Ecology in Health & Disease, 1990
- Protein Degradation by Human Intestinal BacteriaMicrobiology, 1986
- A simple and rapid preparation of alditol acetates for monosaccharide analysisCarbohydrate Research, 1983