Differential Measurement of Cortisol and Cortisone in Human Saliva by HPLC with Uv Detection
- 1 June 1991
- journal article
- research article
- Published by Taylor & Francis Ltd in Journal of Liquid Chromatography
- Vol. 14 (9), 1813-1827
- https://doi.org/10.1080/01483919108049655
Abstract
Both cortisol and its dehydro metabolite cortisone are present in normal human saliva. A method for differential Measurement of both compounds in 1 ml samples of saliva by HPLC/UV is described. the method uses an extraction column having a cyclodextrin bonded phase to retain the compounds of interest while allowing elution of interfering compounds. A steroid-bearing fraction is eluted from the cyclodextrin column, dried, reconstituted in a weak mobile phase, and injected on a reversed phase HPLC/UV system provided with an injector-mounted reversed phase extraction column. Samples containing corticosteroid concentrations as low as 0.5 ng/ml can be effectively analyzed by this method.Keywords
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