Efficient replication of rhesus cytomegalovirus variants in multiple rhesus and human cell types
- 16 December 2008
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 105 (50), 19950-19955
- https://doi.org/10.1073/pnas.0811063106
Abstract
Rhesus cytomegalovirus infection of rhesus macaques has emerged as a model for human cytomegalovirus pathogenesis. The UL128-UL131 locus of the human virus is a primary determinant for viral entry into epithelial cells, an important cell type during cytomegalovirus infection. Rhesus cytomegalovirus strain 68-1 spreads slowly when grown in cultured rhesus epithelial cells, and it does not code for ORFs corresponding to UL128 and the second exon of UL130. We repaired the UL128-UL131 locus of strain 68-1, using rhesus cytomegalovirus strain 180.92 as template, to generate BRh68-1.1. We also repaired a mutation in the UL36 ORF in BRh68-1.1 to make BRh68-1.2. Both repaired derivatives replicate much more efficiently than parental 68-1 virus in rhesus epithelial cells, suggesting that strain 68-1 may be attenuated. Intriguingly, BRh68-1.1 and BRh68-1.2 replicate efficiently in cultured human epithelial cells and endothelial cells. The extended human cell host range of the repaired viruses raises the possibility that rhesus cytomegalovirus-like viruses will be found in humans.This publication has 45 references indexed in Scilit:
- Human Cytomegalovirus Glycoproteins gB and gH/gL Mediate Epithelial Cell-Cell Fusion When Expressed either in cis or in transJournal of Virology, 2008
- A heterologous DNA prime/protein boost immunization strategy for rhesus cytomegalovirusVaccine, 2008
- Suppression of immediate-early viral gene expression by herpesvirus-coded microRNAs: Implications for latencyProceedings of the National Academy of Sciences of the United States of America, 2008
- Protein coding content of the ULb′ region of wild-type rhesus cytomegalovirusVirology, 2008
- Functional Genetic Analysis of Rhesus Cytomegalovirus: Rh01 Is an Epithelial Cell Tropism FactorJournal of Virology, 2008
- Characterization of the Human Cytomegalovirus gH/gL/UL128-131 Complex That Mediates Entry into Epithelial and Endothelial CellsJournal of Virology, 2008
- Human cytomegalovirus uses two distinct pathways to enter retinal pigmented epithelial cellsProceedings of the National Academy of Sciences of the United States of America, 2007
- Cytomegalovirus UL131-128 Products Promote gB Conformational Transition and gB-gH Interaction during Entry into Endothelial CellsJournal of Virology, 2007
- Human Cytomegalovirus Tropism for Endothelial Cells: Not All Endothelial Cells Are Created EqualJournal of Virology, 2007
- Immunogenicity and Protective Efficacy of DNA Vaccines Expressing Rhesus Cytomegalovirus Glycoprotein B, Phosphoprotein 65-2, and Viral Interleukin-10 in Rhesus MacaquesJournal of Virology, 2007