Triptolide inhibits TNF-α, IL-1β and NO production in primary microglial cultures
- 1 May 2003
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in NeuroReport
- Vol. 14 (7), 1091-1095
- https://doi.org/10.1097/00001756-200305230-00037
Abstract
Microglia are believed to participate in the mediation of neurodegeneration through producing a variety of cytotoxic factors upon activation. Pharmacological intervention in microglial activation may therefore exert a neuroprotective effect. In exploring pharmacological agents that can affect microglial activation, we found in this study that triptolide possesses a powerful inhibitory influence over microglia. Pretreatment with triptolide was able to dose-dependently reduce the lipopolysaccharide (LPS)-induced nitrite accumulation and tumor necrosis factor-α and interleukin-1β release from LPS-activated microglia as revealed by Griess reaction and ELISA, respectively. Triptolide reduced LPS-stimulated mRNA expression of all three inflammatory factors. The results obtained from this study demonstrate that triptolide can inhibit inflammatory responses of microglia to inflammatory stimulation via a mechanism involving the inhibition of the synthesis and release of inflammatory factors.Keywords
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