Primary Structure and Antibacterial Activity of Chicken Bone Marrow-Derived β-Defensins
- 1 November 2009
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 53 (11), 4647-4655
- https://doi.org/10.1128/aac.00301-09
Abstract
Three biologically active β-defensins were purified by chromatography from chicken bone marrow extract: avian β-defensin 1 (AvBD1), AvBD2, and the newly isolated β-defensin AvBD7. Mass spectrometry analyses showed that bone marrow-derived AvBD1, -2, and -7 peptides were present as mature peptides and revealed posttranslational modifications for AvBD1 and AvBD7 in comparison to their in silico-predicted amino acid sequences. Tandem mass spectrometry analysis using the nanoelectrospray-quadrupole time of flight method showed N-terminal glutaminyl cyclization of mature AvBD7 and C-terminal amidation of mature AvBD1 peptide, while posttranslational modifications were absent in bone marrow-derived mature AvBD2 peptide. Furthermore, mass spectrometry analysis performed on intact cells confirmed the presence of these three peptides in mature heterophils. In addition, the antibacterial activities of the three β-defensins against a large panel of gram-positive and -negative bacteria were assessed. While the three defensins displayed similar antibacterial spectra of activity against gram-positive strains, AvBD1 and AvBD7 exhibited the strongest activity against gram-negative strains in comparison to AvBD2.This publication has 49 references indexed in Scilit:
- Differential modulation of β-defensin gene expression by Salmonella Enteritidis in intestinal epithelial cells from resistant and susceptible chicken inbred linesDevelopmental & Comparative Immunology, 2009
- Discovery of Anas platyrhynchos avian β-defensin 2 (Apl_AvBD2) with antibacterial and chemotactic functionsMolecular Immunology, 2009
- Evaluation of Strategies for Improving Proteolytic Resistance of Antimicrobial Peptides by Using Variants of EFK17, an Internal Segment of LL-37Antimicrobial Agents and Chemotherapy, 2009
- Glutaminyl cyclases from animals and plants: a case of functionally convergent protein evolutionBiological Chemistry, 2008
- Avian defensinsVeterinary Immunology and Immunopathology, 2008
- Tandem mass spectrometry of amidated peptidesJournal of Mass Spectrometry, 2006
- Mammalian defensins in the antimicrobial immune responseNature Immunology, 2005
- Gallinacins: cysteine‐rich antimicrobial peptides of chicken leukocytesFEBS Letters, 1994
- Ultrasensitive assays for endogenous antimicrobial polypeptidesJournal of Immunological Methods, 1991
- Effects on electrophoretic mobility and antibacterial spectrum of removal of two residues from synthetic sarcotoxin IA and addition of the same residues to cecropin BFEBS Letters, 1988