The tight junction protein ZO-1 and an interacting transcription factor regulate ErbB-2 expression
Open Access
- 2 May 2000
- journal article
- research article
- Published by Springer Science and Business Media LLC in The EMBO Journal
- Vol. 19 (9), 2024-2033
- https://doi.org/10.1093/emboj/19.9.2024
Abstract
Epithelial tight junctions regulate paracellular diffusion and restrict the intermixing of apical and basolateral plasma membrane components. We now identify a Y‐box transcription factor, ZONAB (ZO‐1‐associated nucleic acid‐binding protein), that binds to the SH3 domain of ZO‐1, a submembrane protein of tight junctions. ZONAB localizes to the nucleus and at tight junctions, and binds to sequences of specific promoters containing an inverted CCAAT box. In reporter assays, ZONAB and ZO‐1 functionally interact in the regulation of the ErbB‐2 promoter in a cell density‐dependent manner. In stably transfected overexpressing cells, ZO‐1 and ZONAB control expression of endogenous ErbB‐2 and function in the regulation of paracellular permeability. These data indicate that tight junctions directly participate in the control of gene expression and suggest that they function in the regulation of epithelial cell differentiation.Keywords
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