Dynamics of Microvascular Blood Flow and Oxygenation Measured Simultaneously in Human Skin
- 6 August 2014
- journal article
- clinical trial
- Published by Wiley in Microcirculation
- Vol. 21 (6), 562-573
- https://doi.org/10.1111/micc.12136
Abstract
To evaluate the dynamics of skin microvascular blood flow (BF) and tissue oxygenation parameters (OXY) measured simultaneously at the same site using a combined non-invasive BF+OXY+temperature probe. Skin BF, oxygenated (oxyHb) and deoxygenated (deoxyHb) haemoglobin and mean oxygen saturation (SO2 ) were measured in 50 healthy volunteers at rest and during perturbation of local blood flow by post-occlusive reactive hyperaemia, sympathetic nervous system-mediated vasoconstriction (deep inspiratory breath-hold) and local skin warming. Signals were analysed in time and frequency domains. The relationship between BF and SO2 over the range of flows investigated was described by a non-linear equation with an asymptote for SO2 of 84% at BF >50 PU. SO2 was independently associated with BF, skin temperature, BMI and age, which together identified 59% of the variance in SO2 (p<0.0001). Fourier analysis revealed periodic low frequency fluctuations in both BF and SO2 , attributable to endothelial (~0.01 Hz), neurogenic (~0.04 Hz) and myogenic (~0.1 Hz) flow motion activity. The frequency coherence between the BF and SO2 signals was greatest in the endothelial and neurogenic frequency bands. The simultaneous evaluation of microvascular blood flow and oxygenation kinetics in healthy skin provides a platform from which to investigate microvascular impairment in the skin and more generally the pathogenesis of microvascular disease.Keywords
Funding Information
- Engineering and Physical Sciences Research Council
This publication has 47 references indexed in Scilit:
- Near infrared spectroscopy (NIRS) of the thenar eminence in anesthesia and intensive careAnnals of Intensive Care, 2012
- Noninvasive optical characterization of muscle blood flow, oxygenation, and metabolism in women with fibromyalgiaArthritis Research & Therapy, 2012
- Haemoglobin oxygen saturation as a biomarker: the problem and a solutionPhilosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, 2011
- An association between vasomotion and oxygen extractionAmerican Journal of Physiology-Heart and Circulatory Physiology, 2011
- Investigation of skin vasoreactivity and blood flow oscillations in hypertensive patientsJournal of Hypertension, 2011
- Functional Dilator Capacity is Independently Associated with Insulin Sensitivity and Age in Central Obesity and is not Improved by High Dose Statin TreatmentMicrocirculation, 2010
- Thermal provocation to evaluate microvascular reactivity in human skinJournal of Applied Physiology, 2010
- Evaluation of a new high power, wide separation laser Doppler probe: Potential measurement of deeper tissue blood flowMicrovascular Research, 2009
- Estimation of Coherence Between Blood Flow and Spontaneous EEG Activity in NeonatesIEEE Transactions on Biomedical Engineering, 2005
- The Cutaneous MicrocirculationJournal of Investigative Dermatology Symposium Proceedings, 2000