Evidence of Estrogen Receptors in Normal Human Osteoblast-Like Cells
- 1 July 1988
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 241 (4861), 84-86
- https://doi.org/10.1126/science.3388021
Abstract
In seven strains of cultured normal human osteoblast-like cells, a mean of 1615 molecules of tritium-labeled 17 beta-estradiol per cell nucleus could be bound to specific nuclear sites. The nuclear binding of the labeled steroid was temperature-dependent, steroid-specific, saturable, and cell type-specific. These are characteristics of biologically active estrogen receptors. Pretreatment with 10 nanomolar estradiol in vitro increased the specific nuclear binding of progesterone in four of six cell strains, indicating an induction of functional progesterone receptors. RNA blot analysis demonstrated the presence of messenger RNA for the human estrogen receptor. The data suggest that estrogen acts directly on human bone cells through a classical estrogen receptor-mediated mechanism.Keywords
This publication has 22 references indexed in Scilit:
- 17 beta-estradiol acts directly on the clonal osteoblastic cell line UMR106.Proceedings of the National Academy of Sciences of the United States of America, 1987
- Involutional OsteoporosisThe New England Journal of Medicine, 1986
- Sequence and Expression of Human Estrogen Receptor Complementary DNAScience, 1986
- Cloning of the human oestrogen receptor cDNAJournal of Steroid Biochemistry, 1986
- Human bone cellsin vitroCalcified Tissue International, 1985
- Monoclonal antibodies localize oestrogen receptor in the nuclei of target cellsNature, 1984
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979
- Oestrogen binding proteins in bone cell cytosolCalcified Tissue International, 1978
- LONG-TERM PREVENTION OF POSTMENOPAUSAL OSTEOPOROSIS BY ŒSTROGEN: EVIDENCE FOR AN INCREASED BONE MASS AFTER DELAYED ONSET OF ŒSTROGEN TREATMENTThe Lancet, 1976
- Estrogen-Receptor InteractionScience, 1973