Conformational Rigidity of N4-Acetyl-2′-O-methylcytidine Found in tRNA of Extremely Thermophilic Archaebacteria (Archaea)
- 1 February 1992
- journal article
- research article
- Published by Taylor & Francis Ltd in Nucleosides and Nucleotides
- Vol. 11 (2-4), 759-771
- https://doi.org/10.1080/07328319208021740
Abstract
The conformational characteristics of N 4-acetyl-2′-O-methylcytidine (ac4Cm), which is one of the modified cytidines unique to the tRNA of extremely thermophilic archaebacteria, and related nucleosides, N 4-acetylcytidine (ac4C), 2′-O-methylcytidine (Cm) and cytidine, were analyzed by proton nuclear magnetic resonance spectroscopy. Ribose methylation and N 4-acylation were found to confer high conformational rigidity to the ribose moiety, suggesting that these post-transcriptional modifications play a role in structural stabilization of tRNA, which is of particular importance at high temperature.Keywords
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